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Research ArticleExperimental Studies

Double-layered Nanofibrous Patch for Prevention of Anastomotic Leakage and Peritoneal Adhesions, Experimental Study

JACHYM ROSENDORF, MARKETA KLICOVA, LENKA CERVENKOVA, RICHARD PALEK, JANA HORAKOVA, ANDREA KLAPSTOVA, PETR HOSEK, VLADIMIRA MOULISOVA, LUKAS BEDNAR, VACLAV TEGL, ONDREJ BRZON, ZBYNEK TONAR, VLADISLAV TRESKA, DAVID LUKAS and VACLAV LISKA
In Vivo March 2021, 35 (2) 731-741; DOI: https://doi.org/10.21873/invivo.12314
JACHYM ROSENDORF
1Department of Surgery, Faculty of Medicine in Pilsen, Charles University, Prague, Czech Republic;
2Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Prague, Czech Republic;
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  • For correspondence: jachymrosendorf@gmail.com
MARKETA KLICOVA
3Department of Nonwovens and Nanofibrous Materials, Faculty of Textile Engineering, Technical University of Liberec, Liberec, Czech Republic;
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LENKA CERVENKOVA
2Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Prague, Czech Republic;
4Department of Pathology, Third Faculty of Medicine, Charles University, Prague, Czech Republic;
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RICHARD PALEK
1Department of Surgery, Faculty of Medicine in Pilsen, Charles University, Prague, Czech Republic;
2Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Prague, Czech Republic;
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JANA HORAKOVA
3Department of Nonwovens and Nanofibrous Materials, Faculty of Textile Engineering, Technical University of Liberec, Liberec, Czech Republic;
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ANDREA KLAPSTOVA
3Department of Nonwovens and Nanofibrous Materials, Faculty of Textile Engineering, Technical University of Liberec, Liberec, Czech Republic;
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PETR HOSEK
2Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Prague, Czech Republic;
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VLADIMIRA MOULISOVA
2Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Prague, Czech Republic;
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LUKAS BEDNAR
2Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Prague, Czech Republic;
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VACLAV TEGL
2Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Prague, Czech Republic;
5Department of Anesthesiology and Intensive Care Medicine, Faculty of Medicine in Plzen, Pilsen, Czech Republic;
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ONDREJ BRZON
2Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Prague, Czech Republic;
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ZBYNEK TONAR
2Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Prague, Czech Republic;
6Department of Histology and Embryology, Faculty of Medicine in Pilsen, Charles University, Prague, Czech Republic;
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VLADISLAV TRESKA
1Department of Surgery, Faculty of Medicine in Pilsen, Charles University, Prague, Czech Republic;
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DAVID LUKAS
3Department of Nonwovens and Nanofibrous Materials, Faculty of Textile Engineering, Technical University of Liberec, Liberec, Czech Republic;
7Department of Chemistry, Faculty of Science, Humanities and Education, Technical University of Liberec, Liberec, Czech Republic
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VACLAV LISKA
1Department of Surgery, Faculty of Medicine in Pilsen, Charles University, Prague, Czech Republic;
2Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Prague, Czech Republic;
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    Figure 1.

    Construction of anastomosis. An intestinal anastomosis with defect on the antimesenteric side (I.), the size of the defect is standardized using a drain tube.

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    Figure 2.

    Scanning electron microscopy. Scanning electron microscopy images of the two prepared materials; A) PCL/PVA 1 material; B) PCL/PVA 2 material.

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    Figure 3.

    Intraoperative findings. Examples of intraoperative findings, the forceps point to the anastomosis in all of the specimens; A) animal from Control group, well healed anastomosis, no defect is visible; B) animal from PCL/PVA1 group, the material is visible, a string of omental adhesion is attached to the anastomosis, no defect is visible; C) animal from PCL/PVA2 group, multiple adhesions of the anastomosed intestine, material is visible under a layer of peritoneum and peritoneal adhesions.

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    Figure 4.

    Specimens in blue–trichrome-stained comprehensive histological slides. A) example from the Control group; B) example from the PCL/PVA1 group; C) example from the PCL/PVA2 group.

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    Figure 5.

    Results of histological evaluation in box plot graphs. A) Anastomosis deficiency score; B) comparison of collagen fibers volume fractions; C) comparison of vWF positive cells volume fractions; D) comparison of MAC387 positive cells volume fractions.

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    Figure 6.

    Washed out material imprint. A PCL/PVA1 specimen in blue trichrome staining, the two separated layers of the material are clearly visible.

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In Vivo: 35 (2)
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March-April 2021
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Double-layered Nanofibrous Patch for Prevention of Anastomotic Leakage and Peritoneal Adhesions, Experimental Study
JACHYM ROSENDORF, MARKETA KLICOVA, LENKA CERVENKOVA, RICHARD PALEK, JANA HORAKOVA, ANDREA KLAPSTOVA, PETR HOSEK, VLADIMIRA MOULISOVA, LUKAS BEDNAR, VACLAV TEGL, ONDREJ BRZON, ZBYNEK TONAR, VLADISLAV TRESKA, DAVID LUKAS, VACLAV LISKA
In Vivo Mar 2021, 35 (2) 731-741; DOI: 10.21873/invivo.12314

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Double-layered Nanofibrous Patch for Prevention of Anastomotic Leakage and Peritoneal Adhesions, Experimental Study
JACHYM ROSENDORF, MARKETA KLICOVA, LENKA CERVENKOVA, RICHARD PALEK, JANA HORAKOVA, ANDREA KLAPSTOVA, PETR HOSEK, VLADIMIRA MOULISOVA, LUKAS BEDNAR, VACLAV TEGL, ONDREJ BRZON, ZBYNEK TONAR, VLADISLAV TRESKA, DAVID LUKAS, VACLAV LISKA
In Vivo Mar 2021, 35 (2) 731-741; DOI: 10.21873/invivo.12314
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Keywords

  • Anastomotic leak
  • colorectal surgery
  • anastomotic reinforcement
  • nanofibrous materials
  • polycaprolactone
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