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Research ArticleExperimental Studies

A Novel Procedure for Orthotopic Tibia Implantation for Establishment of a More Clinical Osteosarcoma PDOX Mouse Model

NATHANIEL F. WU, JUN YAMAMOTO, MICHAEL BOUVET and ROBERT M. HOFFMAN
In Vivo January 2021, 35 (1) 105-109; DOI: https://doi.org/10.21873/invivo.12237
NATHANIEL F. WU
1AntiCancer Inc, San Diego, CA, U.S.A.;
2Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, U.S.A.;
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JUN YAMAMOTO
1AntiCancer Inc, San Diego, CA, U.S.A.;
3Department of Surgery, University of California, San Diego, CA, U.S.A.
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MICHAEL BOUVET
3Department of Surgery, University of California, San Diego, CA, U.S.A.
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ROBERT M. HOFFMAN
1AntiCancer Inc, San Diego, CA, U.S.A.;
3Department of Surgery, University of California, San Diego, CA, U.S.A.
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  • For correspondence: all@anticancer.com
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Abstract

Background/Aim: Osteosarcoma is a rare type of malignancy that affects mostly children and adolescents. A new procedure was designed to create an improved patient-derived orthotopic xenograft (PDOX) mouse model of osteosarcoma that more closely mimics osteosarcoma in clinical settings. Previous osteosarcoma PDOX models involved implanting a tumor fragment near the femur of nude mice in a space created by separating muscle. Materials and Methods: A hole was created in the tibia of nude mice and an osteosarcoma tumor fragment was implanted directly into the bone. Results: This procedure resulted in tumor growth in the bone similar to osteosarcoma tumors found in clinical patients. Conclusion: The establishment ratio for this procedure is 80% making it a practical and clinically-relevant model for screening effective therapies for osteosarcoma patients.

  • PDOX
  • patient-derived orthotopic xenograft
  • osteosarcoma
  • tibia implantation

Footnotes

  • Authors’ Contributions

    N.F.W. and J.Y. designed and performed experiments and wrote the paper; R.M.H. gave technical support and conceptual advice. Writing, review, and/or revision of the manuscript: N.F.W., M.B., and R.M.H.

  • This article is freely accessible online.

  • Conflicts of Interest

    N.F.W., J.Y., and RMH are or were unsalaried associates of AntiCancer Inc. which performs contract research with PDOX models. The Authors declare that there are no potential conflicts of interest regarding this study.

  • Funding

    This work was supported in part by a Yokohama City University research grant “KAMOME Project”, and the Robert M. Hoffman Foundation for Cancer Research, both of which had no role in the design, execution, interpretation, or writing of the study.

  • Received November 3, 2020.
  • Revision received November 21, 2020.
  • Accepted November 25, 2020.
  • Copyright© 2021, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved
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In Vivo: 35 (1)
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A Novel Procedure for Orthotopic Tibia Implantation for Establishment of a More Clinical Osteosarcoma PDOX Mouse Model
NATHANIEL F. WU, JUN YAMAMOTO, MICHAEL BOUVET, ROBERT M. HOFFMAN
In Vivo Jan 2021, 35 (1) 105-109; DOI: 10.21873/invivo.12237

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A Novel Procedure for Orthotopic Tibia Implantation for Establishment of a More Clinical Osteosarcoma PDOX Mouse Model
NATHANIEL F. WU, JUN YAMAMOTO, MICHAEL BOUVET, ROBERT M. HOFFMAN
In Vivo Jan 2021, 35 (1) 105-109; DOI: 10.21873/invivo.12237
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Keywords

  • PDOX
  • patient-derived orthotopic xenograft
  • osteosarcoma
  • tibia implantation
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