Abstract
Background/Aim: Osteosarcoma is a rare type of malignancy that affects mostly children and adolescents. A new procedure was designed to create an improved patient-derived orthotopic xenograft (PDOX) mouse model of osteosarcoma that more closely mimics osteosarcoma in clinical settings. Previous osteosarcoma PDOX models involved implanting a tumor fragment near the femur of nude mice in a space created by separating muscle. Materials and Methods: A hole was created in the tibia of nude mice and an osteosarcoma tumor fragment was implanted directly into the bone. Results: This procedure resulted in tumor growth in the bone similar to osteosarcoma tumors found in clinical patients. Conclusion: The establishment ratio for this procedure is 80% making it a practical and clinically-relevant model for screening effective therapies for osteosarcoma patients.
Footnotes
Authors’ Contributions
N.F.W. and J.Y. designed and performed experiments and wrote the paper; R.M.H. gave technical support and conceptual advice. Writing, review, and/or revision of the manuscript: N.F.W., M.B., and R.M.H.
This article is freely accessible online.
Conflicts of Interest
N.F.W., J.Y., and RMH are or were unsalaried associates of AntiCancer Inc. which performs contract research with PDOX models. The Authors declare that there are no potential conflicts of interest regarding this study.
Funding
This work was supported in part by a Yokohama City University research grant “KAMOME Project”, and the Robert M. Hoffman Foundation for Cancer Research, both of which had no role in the design, execution, interpretation, or writing of the study.
- Received November 3, 2020.
- Revision received November 21, 2020.
- Accepted November 25, 2020.
- Copyright© 2021, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved