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Research ArticleExperimental Studies

Ligation Method to Specifically Label a Liver Segment With Indocyanine Green in an Orthotopic Nude-Mouse Liver-Metastasis Model

HIROTO NISHINO, HANNAH M. HOLLANDSWORTH, YOSHIHIKO TASHIRO, JUN YAMAMOTO, SIAMAK AMIRFAKHRI, FILEMONI FILEMONI, NORIHIKO SUGISAWA, ROBERT M. HOFFMAN and MICHAEL BOUVET
In Vivo November 2020, 34 (6) 3159-3162; DOI: https://doi.org/10.21873/invivo.12150
HIROTO NISHINO
1Department of Surgery, University of California San Diego, San Diego, CA, U.S.A.
2AntiCancer, Inc., San Diego, CA, U.S.A.
3Department of Surgery, Graduate School of Medicine, Kyoto University, Kyoto, Japan
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HANNAH M. HOLLANDSWORTH
1Department of Surgery, University of California San Diego, San Diego, CA, U.S.A.
4Department of Surgery, VA San Diego Healthcare System, San Diego, CA, U.S.A.
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YOSHIHIKO TASHIRO
1Department of Surgery, University of California San Diego, San Diego, CA, U.S.A.
2AntiCancer, Inc., San Diego, CA, U.S.A.
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JUN YAMAMOTO
1Department of Surgery, University of California San Diego, San Diego, CA, U.S.A.
2AntiCancer, Inc., San Diego, CA, U.S.A.
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SIAMAK AMIRFAKHRI
1Department of Surgery, University of California San Diego, San Diego, CA, U.S.A.
4Department of Surgery, VA San Diego Healthcare System, San Diego, CA, U.S.A.
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FILEMONI FILEMONI
1Department of Surgery, University of California San Diego, San Diego, CA, U.S.A.
4Department of Surgery, VA San Diego Healthcare System, San Diego, CA, U.S.A.
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NORIHIKO SUGISAWA
1Department of Surgery, University of California San Diego, San Diego, CA, U.S.A.
2AntiCancer, Inc., San Diego, CA, U.S.A.
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ROBERT M. HOFFMAN
1Department of Surgery, University of California San Diego, San Diego, CA, U.S.A.
2AntiCancer, Inc., San Diego, CA, U.S.A.
4Department of Surgery, VA San Diego Healthcare System, San Diego, CA, U.S.A.
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MICHAEL BOUVET
1Department of Surgery, University of California San Diego, San Diego, CA, U.S.A.
4Department of Surgery, VA San Diego Healthcare System, San Diego, CA, U.S.A.
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  • For correspondence: mbouvet@ucsd.edu
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    Figure 1.

    Experimental schema. (A) Isolation and ligation of the left Glissonean pedicle. (B) ICG (10 μg) was injected via the tail vein, resulting in labeling of the area with preserved blood flow in the liver.

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    Figure 2.

    Liver ischemia caused by ligation of the Glissonean pedicle. (A) Three days after left-Glissonean-pedicle ligation, necrosis occurred due to ischemia (white arrow). (B) Entire liver after removal. Left Glissonean pedicle ligation is depicted by the yellow arrow. Necrosis is indicated by the white arrow.

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    Figure 3.

    Fluorescence image of the ICG-labeled tumor segment. (A) Five minutes after indocyanine green injection (10 μg) via the tail vein, the 800-nm signal was observed on the right lobe of the liver due to blockage of blood flow into the left lobe of the liver due to Glissonean pedicle-ligation. Liver metastasis is indicated by the arrow. (B) Cranial inversion of the liver in the same mouse as Figure 3A. Arrow: liver-metastatic tumor.

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In Vivo: 34 (6)
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Vol. 34, Issue 6
November-December 2020
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Ligation Method to Specifically Label a Liver Segment With Indocyanine Green in an Orthotopic Nude-Mouse Liver-Metastasis Model
HIROTO NISHINO, HANNAH M. HOLLANDSWORTH, YOSHIHIKO TASHIRO, JUN YAMAMOTO, SIAMAK AMIRFAKHRI, FILEMONI FILEMONI, NORIHIKO SUGISAWA, ROBERT M. HOFFMAN, MICHAEL BOUVET
In Vivo Nov 2020, 34 (6) 3159-3162; DOI: 10.21873/invivo.12150

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Ligation Method to Specifically Label a Liver Segment With Indocyanine Green in an Orthotopic Nude-Mouse Liver-Metastasis Model
HIROTO NISHINO, HANNAH M. HOLLANDSWORTH, YOSHIHIKO TASHIRO, JUN YAMAMOTO, SIAMAK AMIRFAKHRI, FILEMONI FILEMONI, NORIHIKO SUGISAWA, ROBERT M. HOFFMAN, MICHAEL BOUVET
In Vivo Nov 2020, 34 (6) 3159-3162; DOI: 10.21873/invivo.12150
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Keywords

  • indocyanine green
  • liver
  • hepatic segment
  • labeling
  • liver metastasis
  • patient-derived orthotopic xenographt
  • PDOX
  • Glissonean pedicle
  • ligation
  • imaging
  • nude mouse
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