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Research ArticleExperimental Studies

Humanized Fluorescent Tumor-associated Glycoprotein-72 Antibody Selectively Labels Colon-cancer Liver Metastases in Orthotopic Mouse Models

HANNAH M. HOLLANDSWORTH, HIROTO NISHINO, MICHAEL TURNER, SIAMAK AMIRFAKHRI, FILEMONI FILEMONI, ROBERT M. HOFFMAN, PAUL J. YAZAKI and MICHAEL BOUVET
In Vivo September 2020, 34 (5) 2303-2307; DOI: https://doi.org/10.21873/invivo.12042
HANNAH M. HOLLANDSWORTH
1Department of Surgery, University of California San Diego, San Diego, CA, U.S.A.
2Moores Cancer Center, University of California San Diego, San Diego, CA, U.S.A.
3Department of Surgery, VA San Diego Healthcare System, San Diego, CA, U.S.A.
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HIROTO NISHINO
1Department of Surgery, University of California San Diego, San Diego, CA, U.S.A.
4AntiCancer, Inc., San Diego, CA, U.S.A.
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MICHAEL TURNER
1Department of Surgery, University of California San Diego, San Diego, CA, U.S.A.
2Moores Cancer Center, University of California San Diego, San Diego, CA, U.S.A.
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SIAMAK AMIRFAKHRI
1Department of Surgery, University of California San Diego, San Diego, CA, U.S.A.
2Moores Cancer Center, University of California San Diego, San Diego, CA, U.S.A.
3Department of Surgery, VA San Diego Healthcare System, San Diego, CA, U.S.A.
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FILEMONI FILEMONI
1Department of Surgery, University of California San Diego, San Diego, CA, U.S.A.
2Moores Cancer Center, University of California San Diego, San Diego, CA, U.S.A.
3Department of Surgery, VA San Diego Healthcare System, San Diego, CA, U.S.A.
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ROBERT M. HOFFMAN
1Department of Surgery, University of California San Diego, San Diego, CA, U.S.A.
2Moores Cancer Center, University of California San Diego, San Diego, CA, U.S.A.
3Department of Surgery, VA San Diego Healthcare System, San Diego, CA, U.S.A.
4AntiCancer, Inc., San Diego, CA, U.S.A.
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PAUL J. YAZAKI
5Department of Molecular Imaging and Therapy, Beckman Research Institute of the City of Hope, Duarte, CA, U.S.A.
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MICHAEL BOUVET
1Department of Surgery, University of California San Diego, San Diego, CA, U.S.A.
3Department of Surgery, VA San Diego Healthcare System, San Diego, CA, U.S.A.
3Department of Surgery, VA San Diego Healthcare System, San Diego, CA, U.S.A.
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  • For correspondence: mbouvet@ucsd.edu
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    Figure 1.

    Intra-vital imaging of the LS174T colon-cancer cell-line orthotopic liver-metastasis mouse model. (a) Bright-light imaging. (b) Fluorescence imaging; the metastasis is very brightly labeled and tumor margins are distinct with minimal fluorescent-liver signal after administration of 50 μg huCC49-IR800. Representative image from n=5 mice. Mean TLR=7.53 (SD±2.76).

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    Figure 2.

    Intra-vital imaging of LS174T colon-cancer cell-line orthotopic liver-metastasis mouse model. Tumor is very brightly labeled and margins are distinctly fluorescently visualized after administration of 50 μg huCC49-IR800, with minimal liver or intra-abdominal-organ fluorescence signal.

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    Figure 3.

    Ex-vivo imaging of a tumor section. (a) Bright-light imaging of tumor with surrounding liver. Tumor measures approximately 1 cm in diameter. (b) Ex-vivo fluorescence imaging of a metastasis section demonstrating penetration of the huCC49-IR800 antibody throughout the entire depth of the metastasis. (c) H&E staining of liver metastasis visualized using a 2× objective lens. (d) H&E staining of liver metastasis using a 10× objective lens.

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Humanized Fluorescent Tumor-associated Glycoprotein-72 Antibody Selectively Labels Colon-cancer Liver Metastases in Orthotopic Mouse Models
HANNAH M. HOLLANDSWORTH, HIROTO NISHINO, MICHAEL TURNER, SIAMAK AMIRFAKHRI, FILEMONI FILEMONI, ROBERT M. HOFFMAN, PAUL J. YAZAKI, MICHAEL BOUVET
In Vivo Sep 2020, 34 (5) 2303-2307; DOI: 10.21873/invivo.12042

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Humanized Fluorescent Tumor-associated Glycoprotein-72 Antibody Selectively Labels Colon-cancer Liver Metastases in Orthotopic Mouse Models
HANNAH M. HOLLANDSWORTH, HIROTO NISHINO, MICHAEL TURNER, SIAMAK AMIRFAKHRI, FILEMONI FILEMONI, ROBERT M. HOFFMAN, PAUL J. YAZAKI, MICHAEL BOUVET
In Vivo Sep 2020, 34 (5) 2303-2307; DOI: 10.21873/invivo.12042
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Keywords

  • TAG-72
  • fluorescent antibody
  • colorectal cancer orthotopic
  • liver metastasis
  • nude mice
  • infrared fluorescence imaging
  • fluorescence-guided surgery
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