Abstract
Background/Aim: Dermal mesenchymal stem cells (DMSCs) are pluripotent stem cells found in the skin which maintain the thickness of the dermal layer and participate in skin wound healing. Materials and Methods: The MTT assay was performed to detect cell proliferation and cell-cycle progression and cell-surface markers were assessed by flow cytometry. The levels of proteins in related signaling pathways were detected by western blotting assay and the translocation of β-catenin into the nucleus were detected by immunofluorescence. Red oil O staining was performed to examine the differentiational ability of DMSCs. Results: Knockout of PRDX2 inhibited DMSC cell growth, and cell-cycle arrest at G0/G1 phase; p16, p21 and cyclin D1 expression levels in Prdx2 knockout DMSCs were significantly increased. Furthermore, AKT phosphorylation were significantly increased in Prdx2 knockout DMSCs, GSK3β activity were inhibited, result in β-Catenin accumulated in the nucleus. Conclusion: In conclusion, these results demonstrated that PRDX2 plays a pivotal role in regulating the proliferation of DMSCs, and this is closely related to the AKT/glycogen synthase kinase 3 beta/β-catenin signaling pathway.
Footnotes
Authors' Contributions
Y.H.H., M.H.J., T.K. and H.N.S. performed the experiments and wrote the article. Y.H.J., N.N.Y., J.L., Y.Q.Z., Y.D.C., A.G.W., D.S.L., S.U.K., J.S.K. and Y.H.H. performed the data analysis. Y.H.H., T.K., H.N.S. made substantial contributions to conception and design. All Authors read and approved the final article.
This article is freely accessible online.
Funding
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2017R1D1A1B03028188), KRIBB-OGM5201922, and by the grants from the KRIBB Research Initiative Program (KGM5161914, KGM4251913).
Conflicts of Interest
The Authors declare that there are no conflicts of interest in regard to this study.
- Received September 30, 2019.
- Revision received October 23, 2019.
- Accepted October 29, 2019.
- Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved