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Research ArticleExperimental Studies

Inhibitory Effect of Olive Leaf Extract on Obesity in High-fat Diet-induced Mice

YUN-CHAN JUNG, HYUN WOO KIM, BOK KEE MIN, JAE YOUNG CHO, HYO JEONG SON, JA YOUNG LEE, JI-YOUNG KIM, SAE-BOM KWON, QIANG LI and HEE-WOO LEE
In Vivo May 2019, 33 (3) 707-715; DOI: https://doi.org/10.21873/invivo.11529
YUN-CHAN JUNG
1Institute of Research and Development, Chaon Corp., Seongnam, Republic of Korea
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HYUN WOO KIM
1Institute of Research and Development, Chaon Corp., Seongnam, Republic of Korea
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BOK KEE MIN
2Nova K Health Corp., Seoul, Republic of Korea
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JAE YOUNG CHO
2Nova K Health Corp., Seoul, Republic of Korea
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HYO JEONG SON
2Nova K Health Corp., Seoul, Republic of Korea
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JA YOUNG LEE
2Nova K Health Corp., Seoul, Republic of Korea
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JI-YOUNG KIM
2Nova K Health Corp., Seoul, Republic of Korea
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SAE-BOM KWON
2Nova K Health Corp., Seoul, Republic of Korea
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QIANG LI
1Institute of Research and Development, Chaon Corp., Seongnam, Republic of Korea
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  • For correspondence: liqiang8589{at}snu.ac.kr dinks90{at}chaon.kr
HEE-WOO LEE
1Institute of Research and Development, Chaon Corp., Seongnam, Republic of Korea
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  • For correspondence: liqiang8589{at}snu.ac.kr dinks90{at}chaon.kr
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    Figure 1.

    Effects of olive leaf extract on body weight gain, food intake, and food efficiency ratio (FER) of mice fed high-fat diet (HFD). (A) Experimental groups. (B) Body weight change over a 12-week period. (C-E) Body weight gain, food intake, and FER at 12 weeks. Results are presented as the mean±SEM (n=9) (*p<0.05, **p<0.01, N.S.: Not significant, FER=body weight gain for experimental period/food intake for experimental period).

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    Figure 2.

    Effect of olive leaf extract on adipose tissue in HFD-induced mice. (A) Fatty weight of epididymal, perirenal, retroperitoneal, mesenteric, and total weight in each experimental group. (B) Histological analysis of the epididymal adipose tissue from each group was conducted to quantify the size of adipocytes. Results are presented as the mean±SEM (n=9) (*p<0.05, **p<0.01, N.S.: Not significant).

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    Figure 3.

    Effects of olive leaf extract on the levels of serum biochemicals and cytokines in obese mice fed high-fat diet. (A) Serum levels of triglycerides, free fatty acid, glucose, total cholesterol, HDL-cholesterol, LDL+VLDL-cholesterol were measured. (B) Leptin and adiponectin levels were analyzed by ELISA. Results are presented as the mean±SEM (n=9) (*p<0.05, **p<0.01, N.S.: Not significant).

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    Figure 4.

    RT-PCR analysis of gene expression in the epididymal adipose tissue. The expression levels of adipogenesis-related (PPAR, C/EBP) and thermogenesis-related (PGC-1, UPC-1) mRNAs were evaluated by RT-PCR and normalized to that of GAPDH. Results are presented as the mean±SEM (n=9) (*p<0.05, **p<0.01, N.S.: Not significant).

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May-June 2019
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Inhibitory Effect of Olive Leaf Extract on Obesity in High-fat Diet-induced Mice
YUN-CHAN JUNG, HYUN WOO KIM, BOK KEE MIN, JAE YOUNG CHO, HYO JEONG SON, JA YOUNG LEE, JI-YOUNG KIM, SAE-BOM KWON, QIANG LI, HEE-WOO LEE
In Vivo May 2019, 33 (3) 707-715; DOI: 10.21873/invivo.11529

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Inhibitory Effect of Olive Leaf Extract on Obesity in High-fat Diet-induced Mice
YUN-CHAN JUNG, HYUN WOO KIM, BOK KEE MIN, JAE YOUNG CHO, HYO JEONG SON, JA YOUNG LEE, JI-YOUNG KIM, SAE-BOM KWON, QIANG LI, HEE-WOO LEE
In Vivo May 2019, 33 (3) 707-715; DOI: 10.21873/invivo.11529
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Keywords

  • Obesity
  • olive leaf extract
  • fat
  • adipogenesis
  • thermogenesis
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