An Adult Patient with Early Pre-B Acute Lymphoblastic Leukemia with t(12;17)(p13;q21)/ZNF384-TAF15

A: FISH using ETV6 break-apart probe showing two fusion signals (green and orange signals represent 5’ and 3’ ETV6 gene positions); a hybridization pattern showing absence of ETV6 gene rearrangement. B, C: FISH using ETV6 break-apart probe on a metaphase nucleus stained with DAPI (B) and inverted DAPI. Red and purple arrows show ETV6 fusion signal on derivative chromosome 12 and normal chromosome 12, respectively. In Figure 2C, shows that ETV6 gene is not rearranged and remains on chromosome 12, suggesting that chromosomal breakage has occurred downstream of ETV6 with a direction towards the 12p telomere. D: FISH on a nucleus using TAF15 break-apart probe showing one fusion and one split signal, indicative of TAF15 gene rearrangement. E: FISH on a nucleus using ZNF384 break-apart probe showing one fusion and one split signal, indicative of ZNF384 gene rearrangement.