Article Figures & Data
Figures
- Figure 1.
The chemical structures of β-carotene and lycopene.
- Figure 2.
Stimulation of heme oxygenase 1 (Hmox1) gene expression by β-carotene and lycopene in RAW264.7 cells. The cells were incubated for 3.5 h with the indicated doses of β-carotene or lycopene, and then their total RNAs were prepared. Each cDNA was synthesized, and the expression level of Hmox1 mRNA was determined by real-time polymerase chain reaction and standardized against the expression of 18s rRNA. The results are presented as means±standard error (SE) of three independent experiments, SE<15%. Significant differences between samples for β-carotene and lycopene were observed for stimulation of Hmox1 gene expression. *Significantly different at p<0.01 vs. control group.
- Figure 3.
Stimulation of heme oxygenase 1 (Hmox1) gene expression by tri-n-butyl borane (TBB) in RAW264.7 cells. The cells were incubated for 3.5 h with the indicated doses of TBB, and then their total RNAs were prepared. Each cDNA was synthesized, and the expression level of Hmox1 mRNA was determined by real-time polymerase chain reaction and standardized against the expression of 18s rRNA. The results are presented as means±standard error (SE) of three independent experiments, SE<15%. Significant differences between samples for each TBB were observed for stimulation of Hmox1 gene expression. *Significantly different at p<0.01 vs. control group.
- Figure 4.
Inhibitory effects of β-carotene and lycopene on lipopolysaccharide (LPS)-stimulated cyclooxygenase-2 (Cox2) (A), nitric oxide synthase 2 (Nos2) (B) and tumor necrosis factor-alpha (Tnfa) (C) gene expression in RAW264.7 cells. The cells were pretreated for 30 min with the indicated doses of β-carotene or lycopene. They were then incubated for 3 h with or without LPS at 100 ng/ml, and their total RNAs were prepared. Each cDNA was synthesized, and the expression level of Cox2, Nos2 and Tnfa mRNAs were determined by real-time polymerase chain reaction and standardized against the expression of 18s rRNA. The results are presented as means±standard error (SE) of three independent experiments, SE<15%. Significant differences between samples for β-carotene and lycopene were observed for inhibition of Cox2, Nos2 and Tnfa gene expression. *Significantly different at p<0.01 vs. control group.
- Figure 5.
Inhibitory effects of tri-n-butyl borane (TBB) on lipopolysaccharide (LPS)-stimulated cyclooxygenase-2 (Cox2) (A), nitric oxide synthase 2 (Nos2) (B) gene expression in RAW264.7 cells. The cells were pretreated for 30 min with the indicated doses of TBB. They were then incubated for 3 h with or without LPS at 100 ng/ml, and their total RNAs were prepared. Each cDNA was synthesized, and the expression level of Cox2 and Nos2 mRNAs were determined by real-time polymerase chain reaction and standardized against the expression of 18s rRNA. The results are presented as means±standard error (SE) of three independent experiments, SE<15%. Significant differences between samples for each TBB were observed for inhibition of Cox2 and Nos2 gene expression. *Significantly different at p<0.01 vs. control group.
