Skip to main content

Main menu

  • Home
  • Current Issue
  • Archive
  • Info for
    • Authors
    • Editorial Policies
    • Advertisers
    • Editorial Board
  • Other Publications
    • Anticancer Research
    • Cancer Genomics & Proteomics
    • Cancer Diagnosis & Prognosis
  • More
    • IIAR
    • Conferences
  • About Us
    • General Policy
    • Contact
  • Other Publications
    • In Vivo
    • Anticancer Research
    • Cancer Genomics & Proteomics

User menu

  • Register
  • Subscribe
  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
In Vivo
  • Other Publications
    • In Vivo
    • Anticancer Research
    • Cancer Genomics & Proteomics
  • Register
  • Subscribe
  • My alerts
  • Log in
  • My Cart
In Vivo

Advanced Search

  • Home
  • Current Issue
  • Archive
  • Info for
    • Authors
    • Editorial Policies
    • Advertisers
    • Editorial Board
  • Other Publications
    • Anticancer Research
    • Cancer Genomics & Proteomics
    • Cancer Diagnosis & Prognosis
  • More
    • IIAR
    • Conferences
  • About Us
    • General Policy
    • Contact
  • Visit iiar on Facebook
  • Follow us on Linkedin
Research ArticleExperimental Studies

Anti-inflammatory Activity of β-Carotene, Lycopene and Tri-n-butylborane, a Scavenger of Reactive Oxygen Species

AKIFUMI KAWATA, YUKIO MURAKAMI, SEIJI SUZUKI and SEIICHIRO FUJISAWA
In Vivo March 2018, 32 (2) 255-264;
AKIFUMI KAWATA
Division of Oral Diagnosis and General Dentistry, Department of Diagnostic and Therapeutic Sciences, Meikai University School of Dentistry, Sakado, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
YUKIO MURAKAMI
Division of Oral Diagnosis and General Dentistry, Department of Diagnostic and Therapeutic Sciences, Meikai University School of Dentistry, Sakado, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: ymura@dent.meikai.ac.jp
SEIJI SUZUKI
Division of Oral Diagnosis and General Dentistry, Department of Diagnostic and Therapeutic Sciences, Meikai University School of Dentistry, Sakado, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
SEIICHIRO FUJISAWA
Division of Oral Diagnosis and General Dentistry, Department of Diagnostic and Therapeutic Sciences, Meikai University School of Dentistry, Sakado, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Article Figures & Data

Figures

  • Tables
  • Figure 1.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 1.

    The chemical structures of β-carotene and lycopene.

  • Figure 2.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 2.

    Stimulation of heme oxygenase 1 (Hmox1) gene expression by β-carotene and lycopene in RAW264.7 cells. The cells were incubated for 3.5 h with the indicated doses of β-carotene or lycopene, and then their total RNAs were prepared. Each cDNA was synthesized, and the expression level of Hmox1 mRNA was determined by real-time polymerase chain reaction and standardized against the expression of 18s rRNA. The results are presented as means±standard error (SE) of three independent experiments, SE<15%. Significant differences between samples for β-carotene and lycopene were observed for stimulation of Hmox1 gene expression. *Significantly different at p<0.01 vs. control group.

  • Figure 3.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 3.

    Stimulation of heme oxygenase 1 (Hmox1) gene expression by tri-n-butyl borane (TBB) in RAW264.7 cells. The cells were incubated for 3.5 h with the indicated doses of TBB, and then their total RNAs were prepared. Each cDNA was synthesized, and the expression level of Hmox1 mRNA was determined by real-time polymerase chain reaction and standardized against the expression of 18s rRNA. The results are presented as means±standard error (SE) of three independent experiments, SE<15%. Significant differences between samples for each TBB were observed for stimulation of Hmox1 gene expression. *Significantly different at p<0.01 vs. control group.

  • Figure 4.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 4.

    Inhibitory effects of β-carotene and lycopene on lipopolysaccharide (LPS)-stimulated cyclooxygenase-2 (Cox2) (A), nitric oxide synthase 2 (Nos2) (B) and tumor necrosis factor-alpha (Tnfa) (C) gene expression in RAW264.7 cells. The cells were pretreated for 30 min with the indicated doses of β-carotene or lycopene. They were then incubated for 3 h with or without LPS at 100 ng/ml, and their total RNAs were prepared. Each cDNA was synthesized, and the expression level of Cox2, Nos2 and Tnfa mRNAs were determined by real-time polymerase chain reaction and standardized against the expression of 18s rRNA. The results are presented as means±standard error (SE) of three independent experiments, SE<15%. Significant differences between samples for β-carotene and lycopene were observed for inhibition of Cox2, Nos2 and Tnfa gene expression. *Significantly different at p<0.01 vs. control group.

  • Figure 5.
    • Download figure
    • Open in new tab
    • Download powerpoint
    Figure 5.

    Inhibitory effects of tri-n-butyl borane (TBB) on lipopolysaccharide (LPS)-stimulated cyclooxygenase-2 (Cox2) (A), nitric oxide synthase 2 (Nos2) (B) gene expression in RAW264.7 cells. The cells were pretreated for 30 min with the indicated doses of TBB. They were then incubated for 3 h with or without LPS at 100 ng/ml, and their total RNAs were prepared. Each cDNA was synthesized, and the expression level of Cox2 and Nos2 mRNAs were determined by real-time polymerase chain reaction and standardized against the expression of 18s rRNA. The results are presented as means±standard error (SE) of three independent experiments, SE<15%. Significant differences between samples for each TBB were observed for inhibition of Cox2 and Nos2 gene expression. *Significantly different at p<0.01 vs. control group.

Tables

  • Figures
  • Table I.
PreviousNext
Back to top

In this issue

In Vivo
Vol. 32, Issue 2
March-April 2018
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Back Matter (PDF)
  • Ed Board (PDF)
  • Front Matter (PDF)
Print
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word on In Vivo.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Anti-inflammatory Activity of β-Carotene, Lycopene and Tri-n-butylborane, a Scavenger of Reactive Oxygen Species
(Your Name) has sent you a message from In Vivo
(Your Name) thought you would like to see the In Vivo web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
3 + 12 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.
Citation Tools
Anti-inflammatory Activity of β-Carotene, Lycopene and Tri-n-butylborane, a Scavenger of Reactive Oxygen Species
AKIFUMI KAWATA, YUKIO MURAKAMI, SEIJI SUZUKI, SEIICHIRO FUJISAWA
In Vivo Mar 2018, 32 (2) 255-264;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Reprints and Permissions
Share
Anti-inflammatory Activity of β-Carotene, Lycopene and Tri-n-butylborane, a Scavenger of Reactive Oxygen Species
AKIFUMI KAWATA, YUKIO MURAKAMI, SEIJI SUZUKI, SEIICHIRO FUJISAWA
In Vivo Mar 2018, 32 (2) 255-264;
Reddit logo Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Materials and Methods
    • Results
    • Discussion
    • Acknowledgements
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • PDF

Related Articles

  • No related articles found.
  • PubMed
  • Google Scholar

Cited By...

  • Radical-scavenging and Pro-/anti-inflammatory Activity of Tetracycline and Related Phenolic Compounds With or Without Visible Light Irradiation
  • Google Scholar

More in this TOC Section

  • Evaluation of the Relationship Between miRNA-22-3p and Gal-9 Levels in Glioblastoma
  • Metformin Inhibits the Estrogen-mediated Epithelial-Mesenchymal Transition of Ectopic Endometrial Stromal Cells in Endometriosis
  • MCC950 Ameliorates Acute Exogenous Lipoid Pneumonia Induced by Sewing Machine Oil in Rats via the NF-κB/NLRP3 Inflammasome Pathway
Show more Experimental Studies

Similar Articles

Keywords

  • β-Carotene
  • lycopene
  • tri-n-butyl borane
  • LPS
  • RAW264.7 cells
  • Cox2
  • Nos2
  • HMOX1
In Vivo

© 2023 In Vivo

Powered by HighWire