Research ArticleExperimental Studies

Anti-inflammatory Effects of Oct4/Sox2-overexpressing Human Adipose Tissue-derived Mesenchymal Stem Cells

QIANG LI, SEI-MYOUNG HAN, WOO-JIN SONG, SANG-CHUL PARK, MIN-OK RYU and HWA-YOUNG YOUN
In Vivo May 2017, 31 (3) 349-356;

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Figures

  • Figure 1.
    Figure 1.

    Tri-lineage differentiation of hAT-MSCs. (A) hAT-MSCs exhibit a spindle-shaped fibroblastic morphology (100×). (B) Adipocyte staining as seen by Oil Red O (200×). (C) Differentiation into bone, as shown by Alizarin Red S staining (200×). (D) Differentiation into cartilage as shown by Alcian Blue staining (200×).

  • Figure 2.
    Figure 2.

    Expression analysis of Oct4 and Sox2 in Oct4/Sox2-hAT-MSCs. (A) The mRNA expression levels of Oct4 and Sox2 were analyzed by PCR followed by agarose gel electrophoresis. (B) The results were evaluated by real-time PCR. (C) Oct4 protein expression was confirmed by fluorescence microscopy using immunostaining of Oct4/Sox2-hAT-MSCs. The results are representative of three independent experiments (**p<0.01, ***p<0.001).

  • Figure 3.
    Figure 3.

    Conditioned medium-treated RAW264.7 cells. (A) Conditioned medium-treated RAW264.7 cells were incubated for 12 h and stimulated with LPS (200 ng/ml) for 6 h. Total RNA from RAW264.7 cells was isolated. (B) mRNA expression levels of Il-1β, Tnf-α, Il-6 and Il-10 were evaluated by RT-PCR. (C) mRNA expression levels of Il-1β, Tnf-α, Il-6 and Il-10 were evaluated by real-time PCR. The results are representative of three independent experiments (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001).

  • Figure 4.
    Figure 4.

    Sickness scores of mice 20 h after LPS stimulation. (A) Mice were stimulated with a 20 mg/kg dose of LPS; after 3 h, PBS, GFP-hAT-MSCs and Oct4/Sox2-hAT-MSCs were administered to each group. The sickness scores and survival rates were evaluated after 20 h. (B) After 20 h of LPS stimulation, behavioral and health parameters were evaluated. (C) The average of the total value was compared for total sickness scores. (*p<0.05, **p<0.01, ***p<0.001).

  • Figure 5.
    Figure 5.

    In vivo survival rates following stimulation with LPS. Mice were treated with an intraperitoneal injection of LPS (10 mg/kg) and the survival rate for each group was recorded every 4 h. The survival rates are expressed as the percentage of surviving mice over total mice.

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Anti-inflammatory Effects of Oct4/Sox2-overexpressing Human Adipose Tissue-derived Mesenchymal Stem Cells
QIANG LI, SEI-MYOUNG HAN, WOO-JIN SONG, SANG-CHUL PARK, MIN-OK RYU, HWA-YOUNG YOUN
In Vivo May 2017, 31 (3) 349-356;
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