Article Figures & Data
Figures
- Figure 1.
Experimental schedule. One week before experimentation, all groups were fed tap water. After adipose-derived stem cell (ASCs) transplantation, the control group and the group treated with ASCs were supplied with tap water, while the vitamin C-treated groups were supplied with water containing vitamin C (1.5 g/l) for 4 weeks. All mice were then sacrificed and specimens were collected.
- Figure 2.
Establishment of mouse collagenase-induced tendonitis model. Control tendons exhibited histological features of normal tendons, but tendons in collagenase-induced tendonitis exhibited histopathological lesions of tendonitis (loss of architecture, irregular fibers, infiltration of inflammatory cells, abnormal tenocytes with round nuclei and an abundant cytoplasm, and lacuna formation) Gross view: Frontal; histopathology: Hematoxylin and eosin staining, magnification ×400.
- Figure 3.
Gross and microscopic features of tendons. Gross view: The vitamin C depletion groups exhibited more neovascularization, swelling, and mucus secretion than the groups supplied with vitamin C. Histopathology: The control group exhibited worsened lesions, indicated by separation of fibers with loss of bundle demarcation, high cell density, tenocytes with round nuclei, and infiltration of inflammatory cells. Tenocytes with round nuclei and immature collagen fibers were observed in the group treated with vitamin C, but demarcated bundles were maintained. The group treated with adipose-derived stem cells (ASCs) exhibited local tendon healing (upper part). The combination group exhibited nearly normal tendon features, such as well-demarcated collagen bundles, elongated tenocytes, mature collagen fibers, and low cell density. Gross view: Front and lateral views; histopathology: Hematoxylin and eosin staining, magnification ×100.
- Figure 4.
Bonar score: Semi-quantitative tendon pathological scoring system was used to assess tendons in all groups were evaluated for the degree of histopathological change using the Bonar scoring system: 0, normal state, to 12, worst state). N: Negative (control), V: vitamin C supplementation, C: adipose-derived stem cells (ASCs), and VC: vitamin C supplementation and ASCs transplantation. Data are shown as the mean±SD (*p<0.01).
- Figure 5.
Vitamin C levels in serum. Groups supplied with vitamin C exhibited higher vitamin C levels in the serum than groups with limited vitamin C supply. Despite the equal supplementation of vitamin C, the combination group had higher vitamin C levels than did the group supplemented with vitamin C alone. N: Negative control, C: adipose-derived stem cells (ASCs) transplantation, V: vitamin C supplementation, and VC: vitamin C supplementation and ASC transplantation. Data are shown as the mean±SD (*p<0.01).
In this issue
Jump to section
Related Articles
Cited By...
- No citing articles found.