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Research ArticleExperimental Studies

Preservation and Reproduction of Microminipigs by Cloning Technology

SATOKO ENYA, TATSUO KAWARASAKI, MASAYOSHI OTAKE, AKIHISA KANGAWA, HIROHIDE UENISHI, SATOSHI MIKAWA, TAKASHI NISHIMURA, YASUSHI KUWAHAWA and MASATOSHI SHIBATA
In Vivo September 2016, 30 (5) 617-622;
SATOKO ENYA
1Swine and Poultry Department, Shizuoka Prefectural Research Institute of Animal Industry, Swine and Poultry Research Center, Shizuoka, Japan
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  • For correspondence: fujiyork{at}sp-exp.pref.shizuoka.jp
TATSUO KAWARASAKI
2School of Agriculture, Tokai University, Kumamoto, Japan
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MASAYOSHI OTAKE
1Swine and Poultry Department, Shizuoka Prefectural Research Institute of Animal Industry, Swine and Poultry Research Center, Shizuoka, Japan
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AKIHISA KANGAWA
1Swine and Poultry Department, Shizuoka Prefectural Research Institute of Animal Industry, Swine and Poultry Research Center, Shizuoka, Japan
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HIROHIDE UENISHI
3Animal Bioregulation Unit, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, Ibaraki, Japan
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SATOSHI MIKAWA
4Animal Genome Unit, Division of Animal Breeding and Reproduction, Institute of Livestock and Glassland Science, National Agriculture and Food Research Organization, Ibaraki, Japan
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TAKASHI NISHIMURA
5Fuji Micra Inc., Fujinomiya, Shizuoka, Japan
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YASUSHI KUWAHAWA
5Fuji Micra Inc., Fujinomiya, Shizuoka, Japan
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MASATOSHI SHIBATA
1Swine and Poultry Department, Shizuoka Prefectural Research Institute of Animal Industry, Swine and Poultry Research Center, Shizuoka, Japan
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    Figure 1.

    Microminipig clones and their offspring. (A) Cloned male with silver coat colour and straight snout, similar to that from the cell-stock data. The SLA haplotype A/A, Extension locus E/E and Dominant white locus i/i, were also identical to the types from their cell-stock data. (B) Cloned female with silver coat colour and undershot-jaw snout, similar to that from cell-stock data. The SLA haplotype A/A, the Extension locus E/E and the Dominant white locus i/i were also identical to the types from cell-stock data. (C) Offspring with silver coat colour and straight snout, with SLA haplotype A/A, Extension locus E/E and the Dominant white locus i/i.

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Vol. 30, Issue 5
September-October 2016
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Preservation and Reproduction of Microminipigs by Cloning Technology
SATOKO ENYA, TATSUO KAWARASAKI, MASAYOSHI OTAKE, AKIHISA KANGAWA, HIROHIDE UENISHI, SATOSHI MIKAWA, TAKASHI NISHIMURA, YASUSHI KUWAHAWA, MASATOSHI SHIBATA
In Vivo Sep 2016, 30 (5) 617-622;

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Preservation and Reproduction of Microminipigs by Cloning Technology
SATOKO ENYA, TATSUO KAWARASAKI, MASAYOSHI OTAKE, AKIHISA KANGAWA, HIROHIDE UENISHI, SATOSHI MIKAWA, TAKASHI NISHIMURA, YASUSHI KUWAHAWA, MASATOSHI SHIBATA
In Vivo Sep 2016, 30 (5) 617-622;
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Keywords

  • Miniature pig
  • experimental animal
  • SCNT
  • clone
  • offspring
  • full-sib
  • cryopreservation
  • risk
  • inbreeding
  • genetic drift
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