Article Figures & Data
Figures
- Figure 1.
The chemical structures of 2-tert-butyl-4-methoxyphenol (BHA), 2,4,6-tri-tert-butylphenol (TBP), 2, 6-di-tert-butyl-4-methylphenol (BHT) and BHT intermediates. BHT-Q: (BHT-quinone); BHT-QM: (BHT-quinone methide); BHT-CHO; BHT-OOH; SQ: (stilbenequinone).
- Figure 2.
Effect of 2,6-di-tert-butyl-4-methylphenol (BHT), 2-tert-butyl-4-methoxyphenol (BHA) and 2,4,6-tri-tert-butylphenol (TBP) on lipopolysaccharide (LPS)-stimulated expression of the cyclooxygenase-2 (Cox2) gene in RAW264.7 cells. The cells were pre-treated for 30 min with each of these BHT-related compounds alone at 10 μM, or in three combinations at the same concentration. They were then incubated for 3 h with or without LPS at 100 ng/ml, and their total RNAs were then prepared. Each cDNA was synthesized, and the levels of expression of Cox2 mRNA were determined by real-time polymerase chain reaction and standardized against the expression of 18S rRNA. The results are presented as means±SE of three independent experiments. SE<15%. Significantly different at *p<0.05 and **p<0.01 vs. LPS.
- Figure 3.
Effect of 2,6-di-tert-butyl-4-methylphenol (BHT), 2-tert-butyl-4-methoxyphenol (BHA) and 2,4,6-tri-tert-butylphenol (TBP) on lipopolysaccharide (LPS)-stimulated expression of the tumor necrosis factor alpha (Tnfa) gene in RAW264.7 cells. The cells were pretreated for 30 min with these BHT-related compounds alone at 10 μM, or in three combinations at the same concentration. They were then incubated for 3 h with or without LPS at 100 ng/ml, and their total RNAs were then prepared. Each cDNA was synthesized, and the expression levels of Tnfa mRNA were determined by real-time polymerase chain reaction and standardized against the expression of 18S rRNA. The results are presented as means±SE of three independent experiments. SE<15%. Significantly different at *p<0.05 vs. LPS.
- Figure 4.
Effect of 2,6-di-tert-butyl-4-methylphenol (BHT), 2-tert-butyl-4-methoxyphenol (BHA) and 2,4,6-tri-tert-butylphenol (TBP) on Porphyomonas gingivalis fimbria-stimulated expression of the cyclooxygenase-2 (Cox2) gene in RAW264.7 cells. The cells were pretreated for 30 min with these BHT-related compounds alone at 10 μM, or in three combinations at the same concentration. They were then incubated for 3 h with or without the fimbriae at 4 μg/ml, and their total RNAs were then prepared. Each cDNA was synthesized, and the expression levels of Cox2 mRNA were determined by real-time polymerase chain reaction and standardized against the expression of 18S rRNA. The results are presented as means±SE of three independent experiments. SE<15%. Significantly different at *p<0.05, **p<0.01 and ***p<0.005 vs. fimbria.
- Figure 5.
Effect of 2,6-di-tert-butyl-4-methylphenol (BHT), 2-tert-butyl-4-methoxyphenol (BHA) and 2,4,6-tri-tert-butylphenol (TBP) on Porphyomonas gingivalis fimbria-stimulated expression of the tumor necrosis factor alpha (Tnfa) gene in RAW264.7 cells. The cells were pretreated for 30 min with these BHT-related compounds alone at 10 μM, or in three combinations at the same concentration. They were then incubated for 3 h with or without the fimbriae at 4 μg/ml, and their total RNAs were then prepared. Each cDNA was synthesized, and the levels of expression of Tnfa mRNA were determined by real-time polymerase chain reaction and standardized against the expression of 18S rRNA. The results are presented as means±SE of three independent experiments. SE<15%. Significantly different at *p<0.05 and **p<0.01 vs. fimbriae.
- Figure 6.
Synergistic inhibitory effect of 2,6-di-tert-butyl-4-methylphenol (BHT) and 2-tert-butyl-4-methoxyphenol (BHA) on lipopolysaccharide (LPS)-stimulated expression of the cyclooxygenase-2 (Cox2) gene in RAW264.7 cells. The cells were pretreated for 30 min with the indicated doses of these BHT-related compounds. They were then incubated for 3 h with or without LPS at 100 ng/ml, and their total RNAs were then prepared. Each cDNA was synthesized, and the levels of expression of Cox2 mRNA were determined by real-time polymerase chain reaction and standardized against the expression of 18S rRNA. The results are presented as means±SE of three independent experiments. SE<15%. Significantly different at *p<0.05 and **p<0.01 vs. LPS.
- Figure 7.
Synergistic inhibitory effect of 2,6-di-tert-butyl-4-methylphenol (BHT) and 2-tert-butyl-4-methoxyphenol (BHA) on lipopolysaccharide (LPS)-stimulated expression of the tumor necrosis factor alpha (Tnfa) gene in RAW264.7 cells. The cells were pretreated for 30 min with the indicated doses of these BHT-related compounds. They were then incubated for 3 h with or without LPS at 100 ng/ml, and their total RNAs were then prepared. Each cDNA was synthesized, and the levels of expression of Tnfa mRNA were determined by real-time polymerase chain reaction and standardized against the expression of 18S rRNA. The results are presented as means±SE of three independent experiments. SE<15%. Significantly different at *p<0.05 vs. LPS.
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