Skip to main content

Main menu

  • Home
  • Current Issue
  • Archive
  • Info for
    • Authors
    • Editorial Policies
    • Advertisers
    • Editorial Board
  • Other Publications
    • Anticancer Research
    • Cancer Genomics & Proteomics
    • Cancer Diagnosis & Prognosis
  • More
    • IIAR
    • Conferences
  • About Us
    • General Policy
    • Contact
  • Other Publications
    • In Vivo
    • Anticancer Research
    • Cancer Genomics & Proteomics

User menu

  • Register
  • Subscribe
  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
In Vivo
  • Other Publications
    • In Vivo
    • Anticancer Research
    • Cancer Genomics & Proteomics
  • Register
  • Subscribe
  • My alerts
  • Log in
  • My Cart
In Vivo

Advanced Search

  • Home
  • Current Issue
  • Archive
  • Info for
    • Authors
    • Editorial Policies
    • Advertisers
    • Editorial Board
  • Other Publications
    • Anticancer Research
    • Cancer Genomics & Proteomics
    • Cancer Diagnosis & Prognosis
  • More
    • IIAR
    • Conferences
  • About Us
    • General Policy
    • Contact
  • Visit iiar on Facebook
  • Follow us on Linkedin
Research ArticleExperimental Studies

Characterization of Multiple Myeloma Clonal Cell Expansion and Stromal Wnt/β-catenin Signaling in Hyaluronic Acid-based 3D Hydrogel

NARAYANAN K. NARAYANAN, BIN DUAN, JONATHAN T. BUTCHER, AMITABHA MAZUMDER and BHAGAVATHI A. NARAYANAN
In Vivo January 2014, 28 (1) 67-73;
NARAYANAN K. NARAYANAN
1Department of Environmental Medicine, New York University School of Medicine, Tuxedo, NY, U.S.A.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
BIN DUAN
2Department of Biomedical Engineering, Cornell University, Ithaca, NY, U.S.A.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
JONATHAN T. BUTCHER
2Department of Biomedical Engineering, Cornell University, Ithaca, NY, U.S.A.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
AMITABHA MAZUMDER
3Department of Medicine, New York University School of Medicine, New York, NY, U.S.A.
4NYU Cancer Institute, New York University School of Medicine, New York, NY, U.S.A.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
BHAGAVATHI A. NARAYANAN
1Department of Environmental Medicine, New York University School of Medicine, Tuxedo, NY, U.S.A.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: Bhagavathi.narayanan@nyumc.org
  • Article
  • Figures & Data
  • Info & Metrics
  • PDF
Loading

Abstract

Background: Emerging interest on three-dimensional (3D) cell culture models to replace two-dimensional cultures of cancer cells and their xenografts in immunocompromised animal hosts prompted us to investigate the use of new biodegradable gels to recapitulate the physiological conditions of the microenvironment of multiple myeloma (MM) cells. Materials and Methods: In the present study, for the first time, we used a new 3D model of hyaluronic acid (HA)-based hydrogels with difference in their matrix composition and stiffness. Results: We demonstrated that hyaluronic acid (HA)-based hydrogels perfectly accommodate MM cells; confirmed by cell survival, migration, colony forming units and expression of cell adhesion proteins of the Wnt signaling pathways over a period of time. Conclusion: This study provides the first 3D microenvironment data that HA-based hydrogels could provide with a suitable 3D substratum for MM cells to comprehensively analyze phenotypic changes and the influence of bone marrow stromal stem cells on Wnt/β catenin signaling in response to targeted drug treatments.

  • Multiple myeloma (MM)
  • Wnt
  • β-catenin
  • colony forming units (CFU)
  • three-dimensional (3D)
  • methacrylated hyaluronic acid (Me-HA)
  • methacrylated gelatin (Me-Gel)
  • Received October 26, 2013.
  • Revision received November 11, 2013.
  • Accepted November 13, 2013.
  • Copyright © 2014 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved
View Full Text
PreviousNext
Back to top

In this issue

In Vivo: 28 (1)
In Vivo
Vol. 28, Issue 1
January-February 2014
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Back Matter (PDF)
  • Ed Board (PDF)
  • Front Matter (PDF)
Print
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word on In Vivo.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Characterization of Multiple Myeloma Clonal Cell Expansion and Stromal Wnt/β-catenin Signaling in Hyaluronic Acid-based 3D Hydrogel
(Your Name) has sent you a message from In Vivo
(Your Name) thought you would like to see the In Vivo web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
7 + 10 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.
Citation Tools
Characterization of Multiple Myeloma Clonal Cell Expansion and Stromal Wnt/β-catenin Signaling in Hyaluronic Acid-based 3D Hydrogel
NARAYANAN K. NARAYANAN, BIN DUAN, JONATHAN T. BUTCHER, AMITABHA MAZUMDER, BHAGAVATHI A. NARAYANAN
In Vivo Jan 2014, 28 (1) 67-73;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Reprints and Permissions
Share
Characterization of Multiple Myeloma Clonal Cell Expansion and Stromal Wnt/β-catenin Signaling in Hyaluronic Acid-based 3D Hydrogel
NARAYANAN K. NARAYANAN, BIN DUAN, JONATHAN T. BUTCHER, AMITABHA MAZUMDER, BHAGAVATHI A. NARAYANAN
In Vivo Jan 2014, 28 (1) 67-73;
Reddit logo Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Materials and Methods
    • Results
    • Discussion
    • Acknowledgements
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • PDF

Related Articles

  • No related articles found.
  • PubMed
  • Google Scholar

Cited By...

  • No citing articles found.
  • Google Scholar

More in this TOC Section

  • Enhancement of Wound Healing Efficacy by Chitosan-based Hydrocolloid on Sprague Dawley Rats
  • Expression of ALCAM in Clinical Colon Cancer and Relationship With Patients’ Treatment Responses
  • A Pilot Study of the Association VDR Polymorphisms With Primary Hyperparathyroidism
Show more Experimental Studies

Similar Articles

Keywords

  • Multiple myeloma (MM)
  • Wnt
  • β-catenin
  • colony forming units (CFU)
  • three-dimensional (3D)
  • methacrylated hyaluronic acid (Me-HA)
  • methacrylated gelatin (Me-Gel)
In Vivo

© 2023 In Vivo

Powered by HighWire