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Research ArticleExperimental Studies

Anti-UV/HIV Activity of Kampo Medicines and Constituent Plant Extracts

TAKAO KATO, NORIO HORIE, TOMOHIKO MATSUTA, UMEMURA NAOKI, TETSUO SHIMOYAMA, TADAYOSHI KANEKO, TAISEI KANAMOTO, SHIGEMI TERAKUBO, HIDEKI NAKASHIMA, KAORU KUSAMA and HIROSHI SAKAGAMI
In Vivo November 2012, 26 (6) 1007-1013;
TAKAO KATO
1Department of Oral Surgery, Saitama Medical Center, Saitama Medical University, Moroyama, Japan
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  • For correspondence: sakagami{at}dent.meikai.ac.jp takao{at}saitama-med.ac.jp
NORIO HORIE
1Department of Oral Surgery, Saitama Medical Center, Saitama Medical University, Moroyama, Japan
2Division of Pathology, Sakado, Japan
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TOMOHIKO MATSUTA
3Department of Diagnostic and Therapeutic Sciences, and Meikai Pharmaco-Medical Laboratory (MPL), Sakado, Japan
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UMEMURA NAOKI
4Division of Pharmacology, Sakado, Japan
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TETSUO SHIMOYAMA
1Department of Oral Surgery, Saitama Medical Center, Saitama Medical University, Moroyama, Japan
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TADAYOSHI KANEKO
2Division of Pathology, Sakado, Japan
5Department of Oral and Maxillofacial Surgery II, Nihon University School of Dentistry, Tokyo, Japan
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TAISEI KANAMOTO
6St. Marianna University School of Medicine, Kawasaki, Japan
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SHIGEMI TERAKUBO
6St. Marianna University School of Medicine, Kawasaki, Japan
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HIDEKI NAKASHIMA
6St. Marianna University School of Medicine, Kawasaki, Japan
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KAORU KUSAMA
2Division of Pathology, Sakado, Japan
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HIROSHI SAKAGAMI
3Department of Diagnostic and Therapeutic Sciences, and Meikai Pharmaco-Medical Laboratory (MPL), Sakado, Japan
4Division of Pharmacology, Sakado, Japan
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  • For correspondence: sakagami{at}dent.meikai.ac.jp takao{at}saitama-med.ac.jp
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    Figure 1.

    Effect of 10 Kampo medicines on UV-induced cytotoxocity. Near confluent HSC-2 cells were replaced with PBS(−) containing different concentrations of Kampo medicines. The cells were then exposed to UV irradiation, and the viable cell number was determined as described the Materials and Methods. Each value represents the mean±SD of three independent experiments.

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    Figure 2.

    Effect of 25 Kampo medicines constituent plant extracts on UV-induced cytotoxocity. Near confluent HSC-2 cells were replaced with PBS(−) containing different concentrations of plant extracts. The cells were then exposed to UV irradiation, and the viable cell number was determined as described the Materials and Methods. Each value represents the mean±SD of three independent experiments.

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    Figure 3.

    Inhibition of caspase-3/-7 activation by Glycyrrhiza. HSC-2 cells were exposed to UV irradiation (6 J/m2/min, 1 min) or not in PBS containing 0 (control) or 4 mg/ml of Glycyrrhiza. Cells were incubated for a further 6 hours in fresh culture medium without Glycyrrhiza, and caspase-3/-7 activity of the cell lysates was determined by the production of cleaved product of PARP, using western blot analysis.

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Vol. 26, Issue 6
November-December 2012
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Anti-UV/HIV Activity of Kampo Medicines and Constituent Plant Extracts
TAKAO KATO, NORIO HORIE, TOMOHIKO MATSUTA, UMEMURA NAOKI, TETSUO SHIMOYAMA, TADAYOSHI KANEKO, TAISEI KANAMOTO, SHIGEMI TERAKUBO, HIDEKI NAKASHIMA, KAORU KUSAMA, HIROSHI SAKAGAMI
In Vivo Nov 2012, 26 (6) 1007-1013;

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Anti-UV/HIV Activity of Kampo Medicines and Constituent Plant Extracts
TAKAO KATO, NORIO HORIE, TOMOHIKO MATSUTA, UMEMURA NAOKI, TETSUO SHIMOYAMA, TADAYOSHI KANEKO, TAISEI KANAMOTO, SHIGEMI TERAKUBO, HIDEKI NAKASHIMA, KAORU KUSAMA, HIROSHI SAKAGAMI
In Vivo Nov 2012, 26 (6) 1007-1013;
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Keywords

  • glycyrrhizin
  • Kampo medicine
  • UV protection
  • anti-HIV
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