Chrysin, a Natural and Biologically Active Flavonoid, Influences a Murine Leukemia Model In Vivo through Enhancing Populations of T-and B-Cells, and Promoting Macrophage Phagocytosis and NK Cell Cytotoxicity

Representative mice after exposure to chrysin. Thirty male BALB/c mice were individually injected with WEHI-3 cells then randomly separated into three groups. Group I mice were treated with olive oil. Group II mice were treated with chrysin (10 mg/kg) in olive oil. Group III animals were treated with chrysin (50 mg/kg) in olive oil as described in the Materials and Methods. After sacrifice time, the blood and the spleen from all the animal was collected. NS: Not significant.

Chrysin affected the levels of cell markers in white blood cells from leukemic BALB/c mice. All mice were intraperitoneally injected with WEHI-3 cells for 2 weeks, followed by oral treatment with or without chrysin for 3 weeks. Blood was collected from each animal and was analyzed for cell markers (A: CD3; B: CD19; C: CD11b and D: Mac-3) by flow cytometry as described in Materials and Methods. The data are expressed as the mean±S.D. of three experiments (n=10). *p<0.05 Significant by different between control and chrysin-treated groups. NS: Not significant.

Chrysin stimulated phagocytotic activity of peripheral blood mononuclear cells (PBMCs) in leukemia BALB/c mice. Macrophages were isolated from PBMCs (A) and the peritoneal cavity (B) of each leukemic BALB/c mice after exposure to 0, 10 and 50 mg/kg/day of chrysin by oral administration for three weeks. The percentage of phagocytosis of green fluorescent particles (FITC-E.coli.) under chrysin treatment was determined by flow cytometric analysis as described in the Materials and Methods. Each point is mean±S.D (n=10). *p<0.05 Significant when compared with untreated leukemic mice. NS: Not significant.