Figure 1.
The overall UVC-induced and H2O2-induced DNA repair dynamics in earthworm testis cells. A: Cells were irradiated with indicated dosages of UVC (0-6 J/m2) on ice. Then the cells were subjected to slide making immediately after the UVC irradiation. The slides were sham-digested (▵) or digested with UV endonuclease V twice (▴), and then assessed for DNA strand breaks by comet assay. *P<0.05, with versus without UVC irradiation (UV=0); P<0.05, with versus without UV endonuclease V digestion. B: Cells were irradiated with 6 J/m2 UVC and re-incubated for the indicated time (0-6 h) before slide preparation. Then the slides were sham-digested (▵) or digested with UV endonuclease V for twice (▴), and the DNA strand breaks were detected by comet assay. □Cells without UVC irradiation but with UV endonuclease V digestion. *P<0.05, with versus without repair (0 h); P<0.05, with versus without UV endonuclease V digestion. C: Cells were treated with the indicated dosages of H2O2 (0-80 μM) for 0.5 h on ice. Then the cells were subjected to slide making immediately. The slides were sham-digested (▿) or digested with Fpg and endonuclease III (▾), and then examined for their DNA strand breaks by comet assay. *P<0.05, with versus without H2O2 treatment; P<0.05, with versus without Fpg and endonuclease III digestion. D: Cells were treated with 80 μM of H2O2 and re-incubated for the indicated time (0-6 h) before slide preparation. Then the slides were sham-digested (▿) or digested with Fpg and endonuclease III (▾), and then DNA strand breaks were detected by comet assay. □Cells without H2O2 treatment but with endonuclease V digestion. *P<0.05, with versus without H2O2 treatment; P<0.05, with versus without Fpg and endonuclease III digestion.