Figure 4.
In vivo tumorigenicity of subcutaneously injected sorted PC-3 cells. After isolation by flow cytometry, 0.1 ml of PC-3 cells were subcutaneously injected into NOD/SCID mice, with 100, 1,000, 10,000 cells and mixed at a 1:1 ratio with matrigel. Mice were monitored daily to observe palpable tumor. Tumor sizes were measured with calipers and calculated with the formula V=length×width2×π/6. Mice were injected with 100 (A), 1,000 (B) and 10,000 (C) sorted cells.