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Research ArticleExperimental Studies

Novel Microminipig Model of Atherosclerosis by High Fat and High Cholesterol Diet, Established in Japan

NORIAKI MIYOSHI, MASAHISA HORIUCHI, YOSHINORI INOKUCHI, YOKO MIYAMOTO, NAOKI MIURA, SATATOSHI TOKUNAGA, MAKOTO FUJIKI, YASUKATSU IZUMI, HIROAKI MIYAJIMA, RYOICHI NAGATA, KAZUHIRO MISUMI, TORU TAKEUCHI, AKIHIDE TANIMOTO, NOBUHIRO YASUDA, HIROKI YOSHIDA and HIROAKI KAWAGUCHI
In Vivo September 2010, 24 (5) 671-680;
NORIAKI MIYOSHI
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MASAHISA HORIUCHI
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YOSHINORI INOKUCHI
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YOKO MIYAMOTO
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NAOKI MIURA
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SATATOSHI TOKUNAGA
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MAKOTO FUJIKI
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YASUKATSU IZUMI
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HIROAKI MIYAJIMA
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RYOICHI NAGATA
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KAZUHIRO MISUMI
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TORU TAKEUCHI
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AKIHIDE TANIMOTO
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NOBUHIRO YASUDA
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HIROKI YOSHIDA
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HIROAKI KAWAGUCHI
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  • For correspondence: k3038952@kadai.jp
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Abstract

Novel atherosclerotic lesions were induced in the Microminipig (MMP, registered with the Japanese Ministry of Agriculture, Forestry and Fisheries as a novel variety of swine), the smallest pig available for experimental use, by feeding a high fat (12%) and high cholesterol (5%) diet (HFCD) with sodium cholate (SC, 0.7%) (HFCD/SC) for three months. Three MMPs were used: a male fed with normal diet (M-ND), and a male and an ovariectomized female fed with HFCD/SC (M-HFCD/SC and Fx-HFCD/SC). HFCD/SC induced hypercholesterolemia accompanied by an increase in serum total cholesterol (T-Cho), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C) and cholesterol ester (CE) from the first week. Serum levels of T-Cho, LDL-C and CE reached a maximum in two to three weeks, and HDL-C gradually increased during the experimental period (duration). Serum lipoprotein analysis showed a dominant LDL-C fraction, as seen in humans, in all three MMPs. Body weight gain in the MMPs fed with HFCD/SC was greater than in the animal fed with M-ND. At the end of the experiment, computed tomography scans of conscious animals showed increases in subcutaneous and abdominal fat in those fed with HFCD/SC, suggesting the induction of obesity. Atherosclerotic lesions in systemic arteries (including external and internal iliac arteries, abdominal aorta, coronary artery, cerebral arterial circle), fatty changes, and foamy cell infiltration in the liver and spleen were histopathologically observed in the MMPs fed with HFCD/SC. Atherosclerosis and the pathological findings induced by HFCD/SC in MMPs were similar to the pathological changes associated with human atherosclerosis, suggesting that the MMP has the potential to be a suitable animal model for human atherosclerosis.

  • Atherosclerosis
  • cholesterol
  • diet
  • life-style
  • minipig

Footnotes

  • ↵* These Authors contributed equally to this study.

  • Received May 19, 2010.
  • Revision received July 1, 2010.
  • Accepted July 5, 2010.
  • Copyright © 2010 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved
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Novel Microminipig Model of Atherosclerosis by High Fat and High Cholesterol Diet, Established in Japan
NORIAKI MIYOSHI, MASAHISA HORIUCHI, YOSHINORI INOKUCHI, YOKO MIYAMOTO, NAOKI MIURA, SATATOSHI TOKUNAGA, MAKOTO FUJIKI, YASUKATSU IZUMI, HIROAKI MIYAJIMA, RYOICHI NAGATA, KAZUHIRO MISUMI, TORU TAKEUCHI, AKIHIDE TANIMOTO, NOBUHIRO YASUDA, HIROKI YOSHIDA, HIROAKI KAWAGUCHI
In Vivo Sep 2010, 24 (5) 671-680;

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Novel Microminipig Model of Atherosclerosis by High Fat and High Cholesterol Diet, Established in Japan
NORIAKI MIYOSHI, MASAHISA HORIUCHI, YOSHINORI INOKUCHI, YOKO MIYAMOTO, NAOKI MIURA, SATATOSHI TOKUNAGA, MAKOTO FUJIKI, YASUKATSU IZUMI, HIROAKI MIYAJIMA, RYOICHI NAGATA, KAZUHIRO MISUMI, TORU TAKEUCHI, AKIHIDE TANIMOTO, NOBUHIRO YASUDA, HIROKI YOSHIDA, HIROAKI KAWAGUCHI
In Vivo Sep 2010, 24 (5) 671-680;
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