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Research ArticleExperimental Studies

Mutual Interaction of 17β-Estradiol and Progesterone: Electron Emission. Free Radical Effect Studied by Experiments In Vitro

NIKOLA GETOFF, HEIKE SCHITTL, JOHANNES HARTMANN, MARION GERSCHPACHER, SHAOBIN YING, IREN DANIELOVA and JOHANNES C. HUBER
In Vivo July 2010, 24 (4) 535-541;
NIKOLA GETOFF
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  • For correspondence: nikola.getoff{at}univie.ac.at
HEIKE SCHITTL
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JOHANNES HARTMANN
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MARION GERSCHPACHER
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SHAOBIN YING
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IREN DANIELOVA
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JOHANNES C. HUBER
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    Figure 1.

    Emission of electrons (e−aq) by UV irradiation (λ=254 nm) of 1×10−4 mol/l HBC in air-free, aqueous solution (pH ~7.4). The quantum yields of the ejected electrons, Q(e−aq), at the maxima are given as inset I. The pH as a function of the absorbed UV dose is shown in inset II.

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    Figure 2.

    Electron emission (e−aq) by irradiation of air-free, aqueous solution (pH ~7.4) with monochromatic UV light (λ=254 nm) of: A: 1×10−4 mol/l 17βE2 with 3.98×10−4 mol/l HBC and B: 1×10−4 mol/l PRG with 2.72×10−4 mol/l HBC as a function of the absorbed dose (hν/l). The calculated quantum yields, Q(e−aq), are given as insets.

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    Figure 3.

    Emission of electrons (e−aq) by irradiation of air-free, aqueous solution (pH ~7.4) of 1×10−4 mol/l 17βE2 + 1×10−4 mol/l PRG + 6.7×10−4 mol/l HBC with monochromatic UV light (λ=254 nm) as a function of the absorbed dose (hν/l). Inset I: Q(e−aq) values at the peaks; II: pH as a function of UV dose.

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    Figure 4.

    Toxicity (%) to Escherichia coli bacteria (AB 1157) in aerated, aqueous media (pH~7.4) as a function of substrate concentration (mol/l) of HBC (A) and HBC and PRG (B).

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    Figure 5.

    Survival curves: N/N0 ratio as a function of absorbed γ-radiation dose (Gy) of Escherichia coli bacteria (AB1157) in aqueous aerated media (pH~7.4) in the presence of: A: buffer; B: 2.72×10−4 mol/l HBC; C: 1×10−4 mol/l PRG + 2.72×10−4 mol/l HBC; and D: 1×10−4 mol/l PRG + 1×10−4 mol/l 17βE2 + 6.7×10−4 mol/l HBC. Inset: ΔD37 values (Gy), calculated from the corresponding survival curves.

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    Figure 6.

    Survival curves: N/N0 ratio as a function of absorbed γ-ray dose (Gy) of E. coli bacteria (AB1157) in aqueous solution saturated with N2O (pH~7.4) in the presence of: A: buffer; B: 2.72×10−4 mol/l HBC; C: 1×10−4 mol/l PRG + 2.72×10−4 mol/l HBC; and D: 1×10−4 mol/l PRG + 1×10−4 mol/l 17βE2 + 6.7×10−4 mol/l HBC. Inset: ΔD37 values (Gy), calculated from the corresponding survival curves.

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    Figure 7.

    Survival curves: N/N0 ratio as a function of absorbed γ-ray radiation dose (Gy) of Escherichia coli bacteria (AB1157) in aqueous media (pH~7.4), saturated with argon in the presence of: A: buffer; B: 2.72×10−4 mol/l HBC; C 1×10−4 mol/l PRG + 2.72×10−4 mol/l HBC; and D: 1×10−4 mol/l PRG + 1×10−4 mol/l 17βE2 + 6.7×10−4 mol/l HBC. Inset I: ΔD37 values (Gy) of the given systems; II: Survival curves: N/N0 ratio as a function of absorbed radiation dose (Gy) of the same bacterial type in media saturated with argon in the presence of: A: buffer; B: 3.98×10−4 mol/l HBC; C: 1×10−4 mol/l 17βE2 + 3.98×10−4 mol/l HBC and calculated ΔD37 values of the survival curves.

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Vol. 24, Issue 4
July-August 2010
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Mutual Interaction of 17β-Estradiol and Progesterone: Electron Emission. Free Radical Effect Studied by Experiments In Vitro
NIKOLA GETOFF, HEIKE SCHITTL, JOHANNES HARTMANN, MARION GERSCHPACHER, SHAOBIN YING, IREN DANIELOVA, JOHANNES C. HUBER
In Vivo Jul 2010, 24 (4) 535-541;

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Mutual Interaction of 17β-Estradiol and Progesterone: Electron Emission. Free Radical Effect Studied by Experiments In Vitro
NIKOLA GETOFF, HEIKE SCHITTL, JOHANNES HARTMANN, MARION GERSCHPACHER, SHAOBIN YING, IREN DANIELOVA, JOHANNES C. HUBER
In Vivo Jul 2010, 24 (4) 535-541;
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