Research ArticleExperimental Studies

Selective Deficiency of HIF-1α in Myeloid Cells Influences Secondary Intention Wound Healing in Mouse Skin

RICHARD A. OWINGS, MARJAN BOERMA, JUNRU WANG, MAAIKE BERBEE, KEITH R. LADEROUTE, LEE S.F. SODERBERG, EMRE VURAL and MARTIN HAUER JENSEN
In Vivo November 2009, 23 (6) 879-884;

Article Figures & Data

Figures

  • Figure 1.
    Figure 1.

    Polymerase chain reaction (PCR) detection of the Cre recombinase gene. DNA was isolated from tail snips, amplified (see text), and resolved by agarose gel electrophoresis. Of the 12 samples shown, samples 1, 3, 5, and 7-11 show presence of the Cre recombinase gene.

  • Figure 2.
    Figure 2.

    Secondary intention wound healing in myeloid selective HIF-1α KO and WT mice. Wound area of WT and KO mice, as measured with precision digital calipers immediately after wounding (day 0), and on days 3, 5, 7, 9, and 11 after wounding. The rate of healing during the first 3 days was significantly faster in KO mice than in WT mice (F=39.1, p<0.001). Average, SEM; n=9.

  • Figure 3.
    Figure 3.

    Macrophages and neutrophils in granulation tissue from HIF-1α KO and WT mice. Numbers of ED2-positive cells and MPO-positive cells in granulation tissue of WT and KO mice on day 3 after wounding were determined immunohistochemically. No differences were found between WT and KO mice. Average, SEM; n=3-5.

  • Figure 4.
    Figure 4.

    Immunoreactivity for TGF-β, α-SMC actin, and collagen I in HIF-1α KO and WT mice. Representative images of immunohistochemical stainings of dermal specimens from WT and KO mice. In wounds of day 3, TGF-β was found in granulation tissue and in keratinocytes surrounding the wounds; inset: keratinocytes in mitosis (×100 objective). α-SMC actin was found only in vascular SMC and erector pili. Collagen I immunostaining showed no differences in healed wounds of KO and WT on day 15. E: Epithelial layer, ep: erector pili, G: granulation tissue, S: scab, V: blood vessel.

  • Figure 5.
    Figure 5.

    Response of peritoneal macrophages from HIF-1α KO and WT mice to lipopolysaccharide (LPS) and desferrioxamine (DFX). Production of IL-1β and NO by peritoneal exudate macrophages was measured after in vitro stimulation with LPS and/or DFX. Panel A: Macrophages from WT and KO mice showed similar up-regulation of IL-1β. Panel B: Macrophages from KO mice failed to increase NO production in response to LPS or a combination of LPS and DFX, resulting in a significant difference between WT and KO macrophages (*p=0.01 and p=0.02, respectively). Average, SD; n=3.

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Selective Deficiency of HIF-1α in Myeloid Cells Influences Secondary Intention Wound Healing in Mouse Skin
RICHARD A. OWINGS, MARJAN BOERMA, JUNRU WANG, MAAIKE BERBEE, KEITH R. LADEROUTE, LEE S.F. SODERBERG, EMRE VURAL, MARTIN HAUER JENSEN
In Vivo Nov 2009, 23 (6) 879-884;
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