Article Figures & Data
Figures
- Figure 1.
Optimal concentration of LPS for the induction of NO production by RAW264.7 cells. RAW264.7 cells were incubated for 24 hours with the indicated concentrations of LPS, and the viable cell number and extracellular concentration of NO were then measured. Each value represents the mean±SD of triplicate assays.
- Figure 2.
Inhibition by herbal extracts of LPS-stimulated NO production. RAW264.7 cells were incubated for 24 hours with the indicated concentrations of DB, AS or CO in the presence or absence of 0.1 μg/ml LPS and the viable cell number (absorbance at 540 nm determined by MTT method) (circles) and extracellular concentration of NO (determined by Griess Method) (bars) were then measured. Each value represents the mean±SD of three independent experiments.
- Figure 3.
Inhibition by herbal extracts of LPS-stimulated PGE2 production. RAW264.7 cells were incubated for 24 hours with different concentrations of herbal extracts in the presence of 0.1 μg/ml LPS, and then the viable cell number (absorbance at 540 nm determined by MTT method) (circles) and extracellular PGE2 concentration (determined by EIA) (bars) were determined. Each value represents the mean±SD of three independent experiments.
- Figure 4.
Inhibition of iNOS and COX-2 protein expression by herbal extracts in LPS-stimulated RAW264.7 cells. RAW264.7 cells were treated for 24 hours with the indicated concentrations of DB (0-3 mg/ml), AS (0-6 mg/ml) or CO (0-3 mg/ml) in the presence or absence of LPS (0.1 μg/ml) and processed for Western blot analysis for iNOS, COX-2 and actin (internal marker) protein expression. Representative patterns of Western blot analysis of three independent experiments are shown.
- Figure 5.
Inhibition of iNOS and COX-2 mRNA expression by herbal extracts in LPS-stimulated RAW264.7 cells. RAW264.7 cells were treated for 24 hours with the indicated concentrations of DB, AS or CO in the presence or absence of LPS (0.1 μg/ml) and processed for RT-PCR analysis for iNOS, COX-2 and G3PDH (internal marker) mRNA expression. Similar data were obtained in another two experiments.
- Figure 6.
NO radical-scavenging activity of herbal extracts. Each value represents the mean±S.D. of three independent experiments. IC50 values were determined by the extrapolation of the data. The reducing activity of DB, AS and CO against C-PTI was corrected.
