Figure 4.
Rhein affected the levels of associated proteins in cell cycle and apoptosis of SCC-4 cell. Cells were treated with 30 μM rhein for various time periods and were then harvested for total protein isolation determined as described in Materials and Methods. The associated protein expressions (A: p53, p21, p27, cyclin E, thymidylate synthase, Cdk2, cyclin A and Cdc25A; B: Fas, FasL, pro-caspase-8, caspase-8, Bid, tBid; C: cytochrome c, pro-caspase-9, caspase-9, caspase-3, AIF and PARP; D: Bax and Bcl-2; E: ATF-6α, GADD153 and GRP78) were estimated by Western blotting.