Figure 4.
Blocking STAT3 down-regulated anti-apoptosis and cell cycle gene mRNA levels in vivo. After sacrifice, xenografts were collected and the total RNA was isolated using Trizol Reagent, mRNA levels of STAT3 targeted genes, including survivin, bcl-2, bcl-xl, cyclin D1, cyclin E and c-myc were determined using RT-PCR method. The PCR products were electrophoresed and photographed using AlphaEaseFC. Histogram presented the relative expression level of each gene after normalization to its corresponding internal control (*p<0.05).