Juice of Eclipta prostrata Inhibits Cell Migration In Vitro and Exhibits Anti-angiogenic Activity In Vivo

Abstract

Background: The invasion of cancer cells is critical for metastasis. The effects of Eclipta prostrata, a Thai medicinal plant, on invasion, migration and adhesion of cancer cells were investigated and the anti-angiogenic activity in vivo was evaluated. Materials and Methods: In vitro invasion and migration assays were performed in modified Boyden chambers. In vivo anti-angiogenic activity was determined using the chick chorioallantoic membrane (CAM) assay. Results: E. prostrata juice inhibited cancer invasion and migration, without affecting cell adhesion. Cell migration was inhibited in a variety of cancer cell types and in endothelial cells, with IC₅₀ values of 31-70 μg/ml, much lower than the IC₅₀ values for cytotoxicity of 203-1,217 μg/ml for cancer cells and >4,000 μg/ml for endothelial cells. Fifty percent inhibition of angiogenesis by E. prostrata juice was observed at 200 μg/egg. Conclusion: E. prostrata juice inhibited cancer and endothelial cell migration in vitro and also showed in vivo anti-angiogenic activity.