Skip to main content

Main menu

  • Home
  • Current Issue
  • Archive
  • Info for
    • Authors
    • Editorial Policies
    • Advertisers
    • Editorial Board
    • Special Issues 2025
  • Journal Metrics
  • Other Publications
    • Anticancer Research
    • Cancer Genomics & Proteomics
    • Cancer Diagnosis & Prognosis
  • More
    • IIAR
    • Conferences
  • About Us
    • General Policy
    • Contact
  • Other Publications
    • In Vivo
    • Anticancer Research
    • Cancer Genomics & Proteomics

User menu

  • Register
  • Subscribe
  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
In Vivo
  • Other Publications
    • In Vivo
    • Anticancer Research
    • Cancer Genomics & Proteomics
  • Register
  • Subscribe
  • My alerts
  • Log in
  • My Cart
In Vivo

Advanced Search

  • Home
  • Current Issue
  • Archive
  • Info for
    • Authors
    • Editorial Policies
    • Advertisers
    • Editorial Board
    • Special Issues 2025
  • Journal Metrics
  • Other Publications
    • Anticancer Research
    • Cancer Genomics & Proteomics
    • Cancer Diagnosis & Prognosis
  • More
    • IIAR
    • Conferences
  • About Us
    • General Policy
    • Contact
  • Visit iiar on Facebook
  • Follow us on Linkedin
Research ArticleExperimental Studies

Effects of Prepubertal Indole-3-Carbinol Treatment on Development of N-Methyl-N-Nitrosourea-induced Mammary Carcinomas in Female Sprague-Dawley Rats

NAOTO SHIMANO, NORIHISA UEHARA, YASUHIKO KIYOZUKA, NOBUAKI SHIKATA and AIRO TSUBURA
In Vivo November 2007, 21 (6) 983-988;
NAOTO SHIMANO
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
NORIHISA UEHARA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
YASUHIKO KIYOZUKA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
NOBUAKI SHIKATA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
AIRO TSUBURA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: tsubura@takii.kmu.ac.jp
  • Article
  • Info & Metrics
  • PDF
Loading

Abstract

Background: Indole-3-carbinol (I3C), which is present in cruciferous vegetables, has been shown to prevent the development of mammary cancer when administered to adult animals. However, no studies have been reported on the effects of prepubertal short-term I3C treatment on N-methyl-N-nitrosourea (MNU)-induced mammary carcinogenesis. Materials and Methods: Prepubertal female Sprague-Dawley rats were administered the vehicle (Group 1) or I3C (Group 2, 250 mg/kg/day at 15 and 16 days of age; Group 3, 50 mg/kg/day at 15 and 16 days of age; Group 4, 50 mg/kg/day at 15, 16, 29 and 30 days of age; Group 5, 50 mg/kg/day at 29 and 30 days of age). All rats were administered 50 mg/kg MNU at 22 days of age. Rats were sacrificed at 34 weeks of age or when their largest mammary tumor reached a diameter of ≥1 cm. Body weight gain, vaginal opening, estrous cyclicity and mammary carcinogenesis were compared between the groups. Results: Rats administered 250 mg/kg I3C exhibited acute toxicity, and 40% of that group died soon after administration of I3C. There was no significant difference in body weight and relative uterine-ovarian weight of surviving rats between groups at the end of the experiment. However, rats from Group 2 and Group 3 exhibited earlier vaginal opening and prolonged estrous cyclicity, respectively. I3C treatment before and after MNU administration (Group 4) tended to reduce mammary carcinoma incidence (percentage of mammary carcinomas with a diameter of ≥1 cm) and multiplicity (number of all-sized mammary carcinomas per rat), and prolonged the latency (time from MNU administration to point when mammary tumors grew to a diameter of ≥1 cm) compared with the vehicle (control) group. Mammary carcinogenesis was not altered by other I3C treatments. Conclusion: Prepubertal I3C treatment before and after carcinogen exposure appeared to provide an insignificant protection against MNU-induced mammary carcinogenesis.

  • Indole-3-carbinol
  • N-methyl-N-nitrosourea
  • mammary cancer
  • mammary tumor
  • Sprague-Dawley rat
  • prepuberty

Footnotes

  • Received June 7, 2007.
  • Revision received September 5, 2007.
  • Accepted September 17, 2007.
  • Copyright © 2007 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved
PreviousNext
Back to top

In this issue

In Vivo
Vol. 21, Issue 6
November-December 2007
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word on In Vivo.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Effects of Prepubertal Indole-3-Carbinol Treatment on Development of N-Methyl-N-Nitrosourea-induced Mammary Carcinomas in Female Sprague-Dawley Rats
(Your Name) has sent you a message from In Vivo
(Your Name) thought you would like to see the In Vivo web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
2 + 14 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.
Citation Tools
Effects of Prepubertal Indole-3-Carbinol Treatment on Development of N-Methyl-N-Nitrosourea-induced Mammary Carcinomas in Female Sprague-Dawley Rats
NAOTO SHIMANO, NORIHISA UEHARA, YASUHIKO KIYOZUKA, NOBUAKI SHIKATA, AIRO TSUBURA
In Vivo Nov 2007, 21 (6) 983-988;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Reprints and Permissions
Share
Effects of Prepubertal Indole-3-Carbinol Treatment on Development of N-Methyl-N-Nitrosourea-induced Mammary Carcinomas in Female Sprague-Dawley Rats
NAOTO SHIMANO, NORIHISA UEHARA, YASUHIKO KIYOZUKA, NOBUAKI SHIKATA, AIRO TSUBURA
In Vivo Nov 2007, 21 (6) 983-988;
Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • PDF

Related Articles

  • No related articles found.
  • PubMed
  • Google Scholar

Cited By...

  • No citing articles found.
  • Google Scholar

More in this TOC Section

  • Association of Matrix Metalloproteinase-7 Genotypes With Nasopharyngeal Carcinoma Risk
  • Age- and Periodontal Disease Independent Correlation of Salivary Amino Acids
  • Activation of Invariant Natural Killer T Cells Enhances Ischemia-Reperfusion Injury in Steatotic Mouse Livers
Show more Experimental Studies

Similar Articles

In Vivo

© 2025 In Vivo

Powered by HighWire