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Research ArticleExperimental Studies

Baicalein Induces Apoptosis in SCC-4 Human Tongue Cancer Cells via a Ca2+-dependent Mitochondrial Pathway

YUH-TZY LIN, JAI-SING YANG, HUI-JU LIN, TZU-WEI TAN, NOU-YING TANG, JO-HUA CHAING, YUNG-HSIEN CHANG, HSU-FENG LU and JING-GUNG CHUNG
In Vivo November 2007, 21 (6) 1053-1058;
YUH-TZY LIN
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JAI-SING YANG
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HUI-JU LIN
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TZU-WEI TAN
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NOU-YING TANG
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JO-HUA CHAING
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YUNG-HSIEN CHANG
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HSU-FENG LU
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JING-GUNG CHUNG
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  • For correspondence: jgchung@mail.cmu.edu.tw
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Abstract

Background: The effects of baicalein on SCC-4 human tongue cancer cells were examined to better understand its effect on apoptosis and associated possible signal pathways in vitro. Materials and Methods: Apoptosis induction, reactive oxygen species (ROS), cytoplasmic Ca2+, mitochondrial membrane potential (MMP) and caspase-3 activity were analyzed using the flow cytometric assay. Apoptosis-associated proteins, such as p53, BAX, BCL-2, cytochrome c, caspase-3 and -9, EndoG and AIF were determined by Western blotting. Results: Our results showed that baicalein promoted the levels of p53, BAX, cytochrome c, capase-3 and -9 and reduced the level of BCL-2, which were associated with the induction of apoptotic cell death of SCC-4 cells. A release of cytochrome c from mitochondria into cytosol was demonstrated and an activation of caspase-3, which led to the occurrence of apoptosis in SCC-4 cells treated with baicalein as determined by Western blot. In order to understand the role of Ca2+ in the induction of apoptosis, cells were pre-treated with BAPTA (intracellular calcium chelator) and baicalein. It was shown that the MMP was restored, and the level of cytoplasmic Ca2+ suppressed, the proportion of cells undergoing apoptosis was also markedly diminished. Our data suggest that cellular Ca2+ modulates baicalein-induced cell death via a Ca2+-dependent mitochondrial death pathway in SCC-4 cells.

  • Baicalein
  • reactive oxygen species
  • cytochrome c
  • cytoplasmic Ca2+
  • caspase-3
  • mitochondrial death pathway
  • apoptosis
  • SCC-4 cells

Footnotes

  • Received June 21, 2007.
  • Revision received October 4, 2007.
  • Accepted October 16, 2007.
  • Copyright © 2007 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved
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Vol. 21, Issue 6
November-December 2007
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Baicalein Induces Apoptosis in SCC-4 Human Tongue Cancer Cells via a Ca2+-dependent Mitochondrial Pathway
YUH-TZY LIN, JAI-SING YANG, HUI-JU LIN, TZU-WEI TAN, NOU-YING TANG, JO-HUA CHAING, YUNG-HSIEN CHANG, HSU-FENG LU, JING-GUNG CHUNG
In Vivo Nov 2007, 21 (6) 1053-1058;

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Baicalein Induces Apoptosis in SCC-4 Human Tongue Cancer Cells via a Ca2+-dependent Mitochondrial Pathway
YUH-TZY LIN, JAI-SING YANG, HUI-JU LIN, TZU-WEI TAN, NOU-YING TANG, JO-HUA CHAING, YUNG-HSIEN CHANG, HSU-FENG LU, JING-GUNG CHUNG
In Vivo Nov 2007, 21 (6) 1053-1058;
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