Detection of HCV in Bile Duct Epithelium by Laser Capture Microdissection (LCM)

Abstract

Chronic hepatitis C affects 0.3 to 1.5% of the general population worldwide. The estimated total number of newly acquired hepatitis C virus (HCV) infections is 28,000 in the USA, with 10,000 deaths each year resulting from HCV-associated chronic liver disease. Histological examination of liver tissue from chronic HCV infection shows lymphoid aggregates or follicles in the portal triads, focal fatty change and lobular inflammation. Hepatitis-associated bile duct lesion (HBL) is seen in 5-91% of the cases. While the morphological spectrum of HBL has been well described, its pathogenesis in hepatitis C is not known. To this date, evidence supports both the direct injury and immune-mediated mechanisms, but to what extent these mechanisms are involved in the pathogenesis of HBL in chronic hepatitis C remains unclear. Our study showed the presence of HCV in the bile duct epithelium of patients with chronic hepatitis C infection, using the laser capture microdissection technique. These results will enhance our diagnostic capabilities and treatment of chronic hepatitis C infection.