Skip to main content

Main menu

  • Home
  • Current Issue
  • Archive
  • Info for
    • Authors
    • Editorial Policies
    • Advertisers
    • Editorial Board
  • Other Publications
    • Anticancer Research
    • Cancer Genomics & Proteomics
    • Cancer Diagnosis & Prognosis
  • More
    • IIAR
    • Conferences
  • About Us
    • General Policy
    • Contact
  • Other Publications
    • In Vivo
    • Anticancer Research
    • Cancer Genomics & Proteomics

User menu

  • Register
  • Subscribe
  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
In Vivo
  • Other Publications
    • In Vivo
    • Anticancer Research
    • Cancer Genomics & Proteomics
  • Register
  • Subscribe
  • My alerts
  • Log in
  • My Cart
In Vivo

Advanced Search

  • Home
  • Current Issue
  • Archive
  • Info for
    • Authors
    • Editorial Policies
    • Advertisers
    • Editorial Board
  • Other Publications
    • Anticancer Research
    • Cancer Genomics & Proteomics
    • Cancer Diagnosis & Prognosis
  • More
    • IIAR
    • Conferences
  • About Us
    • General Policy
    • Contact
  • Visit iiar on Facebook
  • Follow us on Linkedin
Research Article

Effects of Aspirin on the In Vitro and In Vivo Acetylation of 2-Aminofluorene in Sprague-Dawley Rats

SONG-SHEI LIN, CHUN-SHU YU, JENG-YUAN WU, YEU-SHENG TYAN, TE-CHUN HSIA, WEN-CHUN LIN and JING-GUNG CHUNG
In Vivo March 2005, 19 (2) 475-481;
SONG-SHEI LIN
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
CHUN-SHU YU
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
JENG-YUAN WU
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
YEU-SHENG TYAN
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
TE-CHUN HSIA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
WEN-CHUN LIN
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
JING-GUNG CHUNG
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: jgchung@mail.cmc.edu.tw
  • Article
  • Info & Metrics
  • PDF
Loading

Abstract

The present study was undertaken to determine the effect of acetylsalicylic acid acid on the in vitro N-acetyltransferase (NAT) enzyme activity and in vivo acetylation of 2-aminofluorene in laboratory rats. In the in vitro experiments, cytosols of blood, bladder, colon and liver cells, with or without acetylsalicylic acid co-treatment, showed different percentages of 2-aminofluorene acetylation. The data indicated that there was decreased NAT activity associated with increased acetylsalicylic acid in the cytosol reaction. In the in vitro experiments, values of apparent Km and Vmax decreased by 4% and 21%, respectively, for acetylation of AF in blood NAT, 28% and 31% for acetylation of AF in bladder NAT, 12% and 25% for acetylation of AF in colon NAT, and 50% and 35% for acetylation of AF in liver NAT. In the in vivo experiments, pretreatment with acetylsalicylic acid (50 mg/kg) 48 h prior to the administration of 2-aminofluorene (50 mg/kg) resulted in 24% and 28% decreases in the fecal and urinary recovery of N-acetyl-2-aminofluorene and a 26% decrease in the metabolic clearance of 2-aminofluorene to N-acetyl-2-aminofluorene. This is the first demonstration of acetylsalicylic acid (Aspirin) inhibition of arylamine N-acetyltransferase activity showing decreases in the N-acetylation of carcinogens in vivo.

Keywords:
  • Acetylsalicylic acid
  • 2-aminofluorene
  • N-acetyl-2-aminofluorene
  • N-acetyltransferase
  • p-aminobenzoic acid
  • N-acetyl-p-aminobenzoic acid

Footnotes

    • Received June 30, 2004.
    • Revision received January 25, 2005.
    • Accepted February 2, 2005.
  • Copyright © 2005 International Institute of Anticaner Research (Dr. John G. Delinassios), All rights reserved
PreviousNext
Back to top

In this issue

In Vivo
Vol. 19, Issue 2
March-April 2005
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for your interest in spreading the word on In Vivo.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Effects of Aspirin on the In Vitro and In Vivo Acetylation of 2-Aminofluorene in Sprague-Dawley Rats
(Your Name) has sent you a message from In Vivo
(Your Name) thought you would like to see the In Vivo web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
12 + 3 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.
Citation Tools
Effects of Aspirin on the In Vitro and In Vivo Acetylation of 2-Aminofluorene in Sprague-Dawley Rats
SONG-SHEI LIN, CHUN-SHU YU, JENG-YUAN WU, YEU-SHENG TYAN, TE-CHUN HSIA, WEN-CHUN LIN, JING-GUNG CHUNG
In Vivo Mar 2005, 19 (2) 475-481;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Reprints and Permissions
Share
Effects of Aspirin on the In Vitro and In Vivo Acetylation of 2-Aminofluorene in Sprague-Dawley Rats
SONG-SHEI LIN, CHUN-SHU YU, JENG-YUAN WU, YEU-SHENG TYAN, TE-CHUN HSIA, WEN-CHUN LIN, JING-GUNG CHUNG
In Vivo Mar 2005, 19 (2) 475-481;
Reddit logo Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • PDF

Related Articles

  • No related articles found.
  • PubMed
  • Google Scholar

Cited By...

  • The Contrasting Role of the Mediator Subunit MED30 in the Progression of Bladder Cancer
  • Identification and Characterization of Functional Rat Arylamine N-Acetyltransferase 3: Comparisons with Rat Arylamine N-Acetyltransferases 1 and 2
  • Google Scholar

Similar Articles

In Vivo

© 2023 In Vivo

Powered by HighWire