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Research Article

N-Acetyltransferase is Involved in Baicalein-induced N-Acetylation of 2-Aminofluorene and DNA-2-Aminofluorene Adduct Formation in Human Leukemia HL-60 Cells

YU-CHING LI, YU-SHENG TYAN, YIH-MIN LEE, TANG-YI TSAO, JING-YUAN CHUANG, HSIU-MAAN KUO, TE-CHUN HSIA, JEN-HUNG YANG and JING-GUNG CHUNG
In Vivo March 2005, 19 (2) 399-405;
YU-CHING LI
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YU-SHENG TYAN
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YIH-MIN LEE
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TANG-YI TSAO
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JING-YUAN CHUANG
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HSIU-MAAN KUO
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TE-CHUN HSIA
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JEN-HUNG YANG
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JING-GUNG CHUNG
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  • For correspondence: jgchung@mail.cmu.edu.tw
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Abstract

Many arylamine and hydrazine drugs are acetylated by cytosolic N-acetyltransferase (NAT). The human promyelocytic leukemia cell line (HL-60) has been shown to acetylate arylamine and contain NAT activity. The purpose of this study was to determine whether or not baicalein could affect N-acetylation of 2-aminofluorene (AF) in HL-60 cells. Acetylated and nonacetylated AF were determined by using high performance liquid chromatography. Baicalein displayed a dose-dependent inhibition of cytosolic and intact cells' NAT activity and reduced the number of viable cells. Time-course experiments showed that N-acetylation of AF, measured from intact HL-60 cells, was inhibited by baicalein for up to 48 h. Baicalein also decreased AF-DNA adduct formation in the examined cells. The effects of baicalein on NAT were examined by flow cytometry and NAT gene expression was examined by polymerase chain reaction. The results demonstrated that baicalein inhibited NAT1 mRNA gene expression and reduced the level of NAT in HL-60 cells. These results show that baicalein can affect the NAT activity of human leukemia cells in vitro.

Keywords:
  • N-acetyltransferase
  • baicalein
  • leukemia cells
  • arylamine
  • 2-aminofluorene

Footnotes

    • Received April 16, 2004.
    • Revision received December 27, 2004.
    • Accepted January 12, 2005.
  • Copyright © 2005 International Institute of Anticaner Research (Dr. John G. Delinassios), All rights reserved
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In Vivo: 19 (2)
In Vivo
Vol. 19, Issue 2
March-April 2005
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N-Acetyltransferase is Involved in Baicalein-induced N-Acetylation of 2-Aminofluorene and DNA-2-Aminofluorene Adduct Formation in Human Leukemia HL-60 Cells
YU-CHING LI, YU-SHENG TYAN, YIH-MIN LEE, TANG-YI TSAO, JING-YUAN CHUANG, HSIU-MAAN KUO, TE-CHUN HSIA, JEN-HUNG YANG, JING-GUNG CHUNG
In Vivo Mar 2005, 19 (2) 399-405;

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N-Acetyltransferase is Involved in Baicalein-induced N-Acetylation of 2-Aminofluorene and DNA-2-Aminofluorene Adduct Formation in Human Leukemia HL-60 Cells
YU-CHING LI, YU-SHENG TYAN, YIH-MIN LEE, TANG-YI TSAO, JING-YUAN CHUANG, HSIU-MAAN KUO, TE-CHUN HSIA, JEN-HUNG YANG, JING-GUNG CHUNG
In Vivo Mar 2005, 19 (2) 399-405;
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