Abstract
We investigated the anti-metastatic effect of cordycepin (3'-deoxyadenosine) on a hematogenic metastatic mouse model which was intravenously injected with B16-BL6 melanoma cells. A 3-hour exposure to various concentrations of cordycepin (0.3, 1 and 3 μg/ml) dose-dependently reduced the number of nodules formed in lung at 15 days after the tumor injection. To elucidate the mechanism of this anti-metastatic effect, we examined the effect of cordycepin on the invasiveness of B16-BL6 cells using a chemo-invasion chamber in vitro. The B16-BL6 cells pretreated with cordycepin (3 μg/ml) for 3 hours showed a significant decrease in invasiveness. Under the same conditions, however, cordycepin did not influence the growth curve of B16-BL6 cells at concentrations up to 3 μg/ml. These results suggest that cordycepin exerts an anti-metastatic action, in part, by inhibiting the invasiveness of mouse melanoma cells.
- Cordycepin (3'-deoxyadenosine)
- anti-metastasis
- B16-BL6 mouse melanoma cells
- hematogenic lung metastatic mouse model
- chemo-invasion
Footnotes
- Received July 5, 2004.
- Accepted October 20, 2004.
- Copyright © 2005 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved